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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Plant Sci. | doi: 10.3389/fpls.2019.01493

Molecular genetic mapping of two complementary genes underpinning fruit bitterness in the bottle gourd [Lagenaria siceraria (Mol.) Standl.]

Xiaohua Wu1,  Xinyi Wu1, Ying Wang1, Baogen Wang1, Zhongfu Lu1,  Pei Xu1 and  Guojing Li1*
  • 1ZheJiang Academy of Agricultural Sciences, China

Fruit bitterness is a serious problem threatening the bottle gourd [Lagenaria siceraria (Mol.) Standl.] industry worldwide. Previous genetic studies indicated that fruit bitterness in the bottle gourd was controlled by a pair of complementary genes. In this study, based on two non-bitter landraces "Hangzhou Gourd" and "Puxian Gourd", each of which carries a single bitterness gene, and their derived segregation populations, we mapped the complementary genes causing fruit bitterness. QTL scanning based on an F2 population detected two QTLs, which was QBt.1 locating in a 17.62 cM interval on LG2 corresponding to a 1.6 Mb region on chromosome 6, and QBt.2 mapped to a 8.44 cM interval on LG9 corresponding to a 1.9 Mb region on chromosome 7. An advanced bulked segregant analysis (A-BSA) well validated the QTL mapping results. Sequence-based comparative analysis showed no syntenic relationship between QBt.1/QBt.2 and the known bitterness genes in cucumber, melon and watermelon, suggesting that causal genes underlying QBt.1 and QBt.2 were not direct orthologs of the reported cucurbit bitterness genes. Our results shed light on the molecular genetic mechanisms underlying fruit bitterness in the bottle gourd and is useful to guide breeders to properly select parental lines to avoid the occurrence of bitter fruits in breeding programs.

Keywords: Bottle gourd, Fruit bitterness, complementary genes, genetic mapping, comparative analysis

Received: 15 Jun 2019; Accepted: 28 Oct 2019.

Copyright: © 2019 Wu, Wu, Wang, Wang, Lu, Xu and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Mx. Guojing Li, ZheJiang Academy of Agricultural Sciences, Hangzhou, 310000, Zhejiang, China, ligj@mail.zaas.ac.cn