Impact Factor 4.106 | CiteScore 4.47
More on impact ›

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Plant Sci. | doi: 10.3389/fpls.2019.01498

Arabidopsis ZUOTIN RELATED FACTOR1 Proteins Are Required for Proper Embryonic and Post-Embryonic Root Development

 Donghong Chen1, 2, 3*, Qiannan Wang1,  Jing FENG1,  YING RUAN3 and  Wen Hui Shen1*
  • 1UPR2357 Institut de biologie moléculaire des plantes (IBMP), France
  • 2School of Forestry and Biotechnology, Zhejiang Agriculture & Forestry University, China
  • 3College of Bioscience & Biotechnology, Hunan Agricultural University, China

The H2A/UBIQUITIN-binding proteins AtZRF1a/b have been reported as key regulators involved in multiple processes of Arabidopsis plant growth and development. Yet, the cellular and molecular mechanisms underlying the mutant phenotype remain largely elusive. Here we show that loss-of-function of AtZRF1a/b causes defective root elongation and deformed root apical meristem (RAM) organization in seedlings. The premature termination of the primary root in the atzrf1a;atzrf1b double mutant is associated with an advanced onset of endoreduplication and subsequent consumption of reservoir stem cells. Cytological analyses using cell type-specific markers and florescent dyes indicate that AtZRF1a/b are involved in maintenance of proper cell layer organization, determinacy of cell identity, and establishment of auxin gradient and maximum at the root tip. During embryogenesis AtZRF1a/b act dominantly in regulating the maintenance of ground tissue initial cells and production of lateral root cap. Lastly, quantitative RT-PCR analysis shows mis-expression of some key genes involved in regulating cell patterning, cell proliferation and/or hormone pathways. Our results provide important insight into AtZRF1a/b function in cell fate determinacy and in establishment and maintenance of proper stem cell reservoir during embryonic and post-embryonic root development.

Keywords: ZRF1, root meristem, Embryogenesis, cell layer organization, cell identity

Received: 20 Aug 2019; Accepted: 29 Oct 2019.

Copyright: © 2019 Chen, Wang, FENG, RUAN and Shen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Donghong Chen, UPR2357 Institut de biologie moléculaire des plantes (IBMP), Strasbourg, 67084, Alsace, France, donghong.chen@zafu.edu.cn
Prof. Wen Hui Shen, UPR2357 Institut de biologie moléculaire des plantes (IBMP), Strasbourg, 67084, Alsace, France, wen-hui.shen@ibmp-cnrs.unistra.fr