Original Research ARTICLE
Chromosome painting facilitates anchoring reference genome sequence to chromosomes in situ and integrated karyotyping in banana (Musa spp.)
- 1Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Czechia
- 2International Institute of Tropical Agriculture (IITA), Uganda
- 3International Institute of Tropical Agriculture (Tanzania), Tanzania
Oligo painting FISH was established to identify all chromosomes in banana (Musa spp.) and to anchor pseudomolecules of reference genome sequence of Musa acuminata spp. malaccensis ‘DH Pahang’ to individual chromosomes in situ. A total of nineteen chromosome / chromosome-arm specific oligo painting probes were developed and were shown to be suitable for molecular cytogenetic studies in genus Musa. For the first time, molecular karyotypes of diploid M. acuminata spp. malaccensis (A genome), M. balbisiana (B genome) and M. schizocarpa (S genome) from the Eumusa section of Musa, which contributed to the evolution of edible banana cultivars, were established. This was achieved after a combined use of oligo painting probes and a set of previously developed banana cytogenetic markers. The density of oligo painting probes was sufficient to study chromosomal rearrangements on mitotic as well as on meiotic pachytene chromosomes. This advance will enable comparative FISH mapping and identification of chromosomal translocations which accompanied genome evolution and speciation in the family Musaceae.
Keywords: Banana, chromosome identification, fluorescence in situ hybridization, Molecular karyotype, Musa, oligo painting FISH
Received: 29 Jul 2019;
Accepted: 29 Oct 2019.
Copyright: © 2019 Šimoníková, Němečková, Karafiátová, Uwimana, Swennen, Doležel and Hřibová. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Mx. Eva Hřibová, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Prague, Czechia, firstname.lastname@example.org