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Front. Plant Sci. | doi: 10.3389/fpls.2019.01508

Physiological traits for shortening crop duration and improving productivity of greengram (Vigna radiata L. Wilczek) under high temperature

 Partha S. Basu1*,  Aditya P. Garg1,  Sanjeev Gupta1,  Kusum Sharma1, Rakhi Tomar1 and Narendra P. Singh1
  • 1Indian Institute of Pulses Research (ICAR), India

Greengram is an important protein-rich food legume crop. During the reproductive stage, high temperatures cause flower drop, induce male sterility, impair anthesis, and shortens the grain-filling period. Initially, 116 genotypes were evaluated for 3 years in two locations, and based on flowering, biomass, and yield attributes, they were grouped into four major clusters. A panel of 17 contrasting genotypes was selected for their heat tolerance in high-temperature greenhouses. The seedlings of the selected genotypes were exposed to heat shock in the range 37°C–52°C and their recovery after heat shock was assessed at 30°C. The seedlings of EC 398889 turned completely green and rejuvenated, while those of LGG 460 failed to recover, therefore, EC 398889 and LGG 460 were identified as heat-tolerant and heat-sensitive genotypes, respectively. Except for EC 398889, the remaining genotypes could not survive after heat shock. Fresh seeds of EC 398889 and LGG 460 were planted in field and pollen fertility and sucrose-synthase (SuSy) activity in grains were assessed at high temperatures. The pollen germination and SuSy activity were normal even at temperatures beyond 40°C in EC 398889 and high SuSy activity enabled faster grain filling than in LGG 460. The precise phenotyping demonstrated significant differences in the light-temperature response of photosynthesis, chlorophyll fluorescence imaging of quantum yield (Fv/Fm), and electron transport rate (ETR) between heat-tolerant (EC 398889) and heat-sensitive (LGG 460) genotypes. Molecular profiling of selected accessions showed polymorphism with 11 SSR markers and the markers CEDG147, CEDG247, and CEDG044 distinguished tolerant and sensitive groups of accessions.

Keywords: Thermotorance, Cell viability, Chlorophyll Fluorescence, sucrose synthase, Photosynthesis

Received: 30 Mar 2019; Accepted: 30 Oct 2019.

Copyright: © 2019 Basu, Garg, Gupta, Sharma, Tomar and Singh. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Partha S. Basu, Indian Institute of Pulses Research (ICAR), Kanpur, India, psbsu59@gmail.com