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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Plant Sci. | doi: 10.3389/fpls.2019.01510

Overexpression of SlOFP20 in Tomato Affects Plant Growth, Chlorophyll Accumulation and Leaf Senescence

 Shengen Zhou1, Xin Chen1, Fenfen Li1, Panpan Feng1, Guoping Chen1, Gongling Hu1, Qiaoli Xie1* and Zongli Hu1*
  • 1Chongqing University, China

Previous studies have shown that OVATE family proteins (OFPs) participate in various aspects of plant growth and development. How OFPs affect leaf chlorophyll accumulation and leaf senescence has not been reported yet. Here, we found that overexpression of SlOFP20 in tomato not only impacted plant architecture but also enhanced the leaf chlorophyll accumulation and retarded leaf senescence. Gene expression analysis of SlGLK1, SlGLK2 and HY5, encoding transcription factors that are putatively involved in chloroplast development and chlorophyll levels, were significantly up-regulated in SlOFP20-OE lines. Both chlorophyll biosynthesis and degradation genes were distinctly regulated in transgenic plants. Moreover, SlOFP20-OE plants accumulated more starch and soluble sugar than WT plants, indicating that an increased chlorophyll content conferred some higher photosynthetic performance in SlOFP20-OE plants. Furthermore, The levels of leaf senescence-related indexes, such as hydrogen peroxide (H2O2), malondialdehyde (MDA) and antioxidant enzymes activities, were differently altered, too. SlOFP20 overexpression repressed the expression of senescence-related genes, SAG12, RAV1 and WRKY53. Moreover, ABA and ethylene synthesis genes were down-regulated in transgenic lines. These results provide new insights into how SlOFP20 regulates chlorophyll accumulation and leaf senescence.

Keywords: Ovate Family Protein, SlOFP20, Chlorophyll, Sugar metabolism, leaf senescence

Received: 25 Jul 2019; Accepted: 30 Oct 2019.

Copyright: © 2019 Zhou, Chen, Li, Feng, Chen, Hu, Xie and Hu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Qiaoli Xie, Chongqing University, Chongqing, China, qiaolixie@cqu.edu.cn
Prof. Zongli Hu, Chongqing University, Chongqing, China, huzongli71@163.com