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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Plant Sci. | doi: 10.3389/fpls.2019.01551

TabZIP74 acts as a positive regulator in wheat stripe rust resistance and involves root development by mRNA splicing

 Fengtao Wang1, Ruiming Lin1*, Yuanyuan Li1, Pei Wang1, 2, Jing Feng1,  Wanquan Chen1 and Shichang Xu1
  • 1State Key Laboratory for Biology of Plant Diseases and Insect Pests (SKLBPI), Agricultural Information Institute (CAAS), China
  • 2College of Plant Protection, China Agricultural University, China

Basic leucine zipper (bZIP) membrane-bound transcription factors (MTFs) play important roles in regulating plant growth and development, abiotic stress responses and disease resistance. Most bZIP MTFs are key components of signaling pathways in endoplasmic reticulum (ER) stress responses. In this study, a full-length cDNA sequence encoding bZIP MTF, designated TabZIP74, was isolated from a cDNA library of wheat near-isogenic line of Taichung29*6/Yr10 inoculated with an incompatible race CYR32 of Puccinia striiformis f. sp. tritici (Pst). Phylogenic analysis showed that TabZIP74 is highly homologous to ZmbZIP60 in maize and OsbZIP74 in rice. The mRNA of TabZIP74 was predicted to form a secondary structure with two kissing hairpin loops and could be spliced, causing an open reading frame shift immediately before the hydrophobic region to produce a new TabZIP74 protein without the transmembrane domain. Pst infection and the abiotic PEG and ABA treatments lead the TabZIP74 mRNA splicing in wheat seedling leaves, while both spliced and unspliced forms in roots were detected. In confocal microscopic examination, TabZIP74 is mobilized into the nucleus from the membrane of tobacco epidermal cells in response to wounding. Knocking down of TabZIP74 with barley stripe mosaic virus-induced gene silencing (BSMV-VIGS) enhanced wheat seedling susceptibility to stripe rust and decreased drought tolerance and lateral roots of silenced plants. These findings demonstrated that TabZIP74 mRNA is induced to splice when stressed by biotic and abiotic factors, acts as a critically positive regulator for wheat stripe rust resistance and drought tolerance, and is necessary for lateral root development.

Keywords: Common wheat, Puccinia striiformis f. sp. tritici,, bZIP transcription factor, er stress, mRNA splicing, Disease Resistance

Received: 20 Jul 2019; Accepted: 06 Nov 2019.

Copyright: © 2019 Wang, Lin, Li, Wang, Feng, Chen and Xu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Ruiming Lin, State Key Laboratory for Biology of Plant Diseases and Insect Pests (SKLBPI), Agricultural Information Institute (CAAS), Haidian, 100081, Beijing, China, linruiming@caas.cn