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Front. Plant Sci. | doi: 10.3389/fpls.2019.01569

Highly Efficient Nanoscale Analysis of Plant Stomata and Cell Surface using Polyaddition Silicone Rubber

 Yi He1*, Kaiyue Zhou2, Zhemin Wu3, Boxiu Li4,  Junliang Fu2, Chinho Lin5 and  Dean Jiang2
  • 1Zhejiang Agriculture and Forestry University, China
  • 2Zhejiang University, China
  • 3Sir Run Run Shaw Hospital, China
  • 4Second Affiliated Hospital, School of Medicine, Zhejiang University, China
  • 5College of Life Sciences, National Chung Hsing University, Taiwan

Stomata are one of the most important physiological apparatus in higher plants, which control gas exchange and water transpiration. The regulation of stomatal aperture is closely coordinated with photosynthesis, uptake of nutrients, plant growth and development and so on. With the development of electron microscope, the high-resolution images of plant stomata and cell surface can be got on the detached plant tissues by scanning electron microscope. However, this method cannot allow us to rapidly analysis the dynamic variation of plant stomata in situ under nondestructive condition. Here, we show a novel plant surface impression technique (PSIT) that can make us to precisely analysis the plant stomata and cell surface. Using this method, we successfully monitored the dynamic variation of stomata and observed the microstructure of soybean leaf trichomes and dragonfly wing at nano-scale. Our results indicate the PSIT can be widely applied to many fields of research.

Keywords: stomata, Stomatal aperture, Cell surface (plant), plant surface impression technique, Nano-Scale

Received: 16 Aug 2019; Accepted: 08 Nov 2019.

Copyright: © 2019 He, Zhou, Wu, Li, Fu, Lin and Jiang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Yi He, Zhejiang Agriculture and Forestry University, Hangzhou, China, heyi@zju.edu.cn