Alisdair Fernie1
Shin-nosuke Hashida2
Kazuya Yoshimura3
Bertrand Gakière4
Zhonglin Mou5
Pierre Pétriacq6*- 1Department of Molecular Physiology, MPI of Molecular Plant Physiology, Potsdam-Golm, Germany
- 2Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry (CRIEPI), Abiko-shi, Japan
- 3Department of Food and Nutritional Science, College of Bioscience and Biotechnology Chubu University, Kasugai, Japan
- 4Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRAE, Université d’Evry, Université Paris-Diderot, Université Paris-Sud, Sorbonne Paris-Cité, Saclay Plant Sciences, Orsay, France
- 5Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, United States
- 6Université de Bordeaux, INRAE, UMR BFP, Plateforme Bordeaux Metabolome, Villenave d’Ornon, France
Editorial on the Research Topic
NAD Metabolism and Signaling in Plants
Since its discovery more than a century ago and the conferral of several Nobel laureates for ground-breaking works concerning the molecule, nicotinamide adenine dinucleotide (NAD) is recognized as a fascinating metabolic cornerstone for all living organisms (Berger et al., 2004). This ubiquitous pyridine nucleotide (and its phosphorylated relative, NADP) is an energy cofactor and signal-carrying molecule exerting vital functions in plant metabolic pathways and regulatory processes (Hashida et al., 2009; Gakière et al., 2018b; Gakière et al., 2018a). Molecular studies on NAD involved transcript, protein, and metabolite profiling, and revealed a particularly complex signaling network that can impact not only the core biochemistry of the plant (e.g. photosynthesis, respiration, energy), but also responses to various stress, more importantly immune responses (Zhang and Mou, 2009; Pétriacq et al., 2012; Pétriacq et al., 2016; Alferez et al., 2018; Hashida et al., 2018). Remarkably, compelling evidence indicate that NAD can be released into the extracellular space, where it is sensed by cell surface receptors (Wang et al., 2017; Wang et al., 2019). However, the detailed mechanisms by which NAD acts as a regulator of plant physiology are still poorly understood. This Research Topic gathers some cardinal knowledge on the functions of NAD in plants.
Two papers in the issue focus on the key enzyme NAD kinase which phosphorylates NAD(H) forming NADP(H) (Gakière et al., 2018a). The first review by Ishikawa and Kawai-Yamada examined the roles of the enzyme in cyanobacteria—the evolutionary ancestor of land plant chloroplasts which has a far simpler level of compartmentation, in the oxidative pentose phosphate pathway, the glycolysis, and the pyridine nucleotide transhydrogenase proton translocation system. Survey of 72 cyanobacterial genomes revealed that 69 express a typical NAD kinase, raising the intriguing questions how typical NAD kinases contribute to NAD(P)(H) homeostasis in cyanobacteria and how this is regulated in the species containing the atypical NAD kinases. Also intriguing is the genetic identification of one of the latter as a factor linked to fast growth (Ungerer et al., 2018). However, a number of open questions remain, mainly why cyanobacteria need multiple NAD kinases and what the specific roles of the paralogs are. Further biochemical work alongside that on the membrane permeability of NAD(P)H will go a long way to addressing these questions.
Secondly, Tai et al. reviewed the interplay of NAD kinases with calmodulin-mediated calcium signaling. They described the importance of the balance between NADP(H) and NAD(H) providing evidence suggesting that in response to allosteric regulation NAD kinase is a key regulator of the NADP(H)/NAD(H) ratio (Li et al., 2018). They then listed the evidence that calmodulin activates NAD kinase before performing a detailed analysis of wheat calmodulins, including predictions of gene and protein structures alongside subcellular localization and structure function studies. This provided a comprehensive overview of the putative regulatory control these proteins exhibit on NAD kinase (Tai et al.). Next, the review described the feedback regulation by NAD+ derivatives, which is necessary for maintaining the dynamic balance of the cellular metabolites that ensure the normal operation of life activities.
Under sunlight, photosynthetic electron transfer chain is the primary source of NADPH as reducing power for assimilation of CO2 by the Calvin cycle. However, under stress conditions that weaken enzyme activity involved in the Calvin cycle, decline of NADPH usage and NADP+ recycling occurs and photosynthetic electron flow can be overloaded resulting in generation of reactive oxygen species. Thus, the NADP(H) pool size and redox state are crucial for the chloroplastic redox balance. An important fact is that, at the beginning of photosynthesis, NADP+ supply control is dominated by de novo NADP+ synthesis rather than recycling from the Calvin cycle. In addition, the activity of NADP+ synthesis and the NADP pool size varies depending on the light conditions and the ferredoxin-thioredoxin system, by which NADP+ is reduced to NADPH. NAD+ is exclusively produced in the cytosol, although NADP+ is produced at on demand sites (cytosol, chloroplasts, and peroxisomes) by various isoforms of NAD kinase. Therefore, the regulatory mechanism of cytosolic NAD+ supply is also involved in the chloroplastic NADP+ supply control. Hashida and Kawai-Yamada summarized the regulatory mechanisms of NADP+ production, focusing on the interactions, crosstalk, and co-regulation between chloroplasts and the cytoplasm at the level of NAD+ metabolism and molecular transport.
NAD metabolism underlies a number of NAD-processing reactions. To start recycling NAD from nicotinate (NA), plants developed various strategies to overcome NA toxicity. Enzymatic conjugation of NA (e.g. glycosylation, methylation) has been discovered before and current metabolic analysis with much higher resolution and selectivity become available to examine NAD catabolites using mass spectrometry. Liu et al. identified two novel NA conjugates using a novel method, NA N-pentoside and NA N-rhamnoside. Interestingly, they provided quantitative profiles of NAD-related metabolites in 24 plant tissues and biochemically characterized the responsible enzymes for conjugation with NA, being a key advance in our understanding of physiological significance of various NA conjugations.
NAD is also important for crops including fruits that constitute an important part of human diets, providing minerals, vitamins, fibers, and antioxidants that are essential for human health. Understanding of the biosynthetic production of these nutrients has attracted considerable attentions in recent years. Although it is well known that fruit development is associated with dramatic metabolic changes (Decros et al., 2019), how NAD metabolism and signaling are regulated during fruit development remains unclear. Decros et al. tackled this question via investigation of the changes in NAD(P) pools, transcriptome, and proteome profiles during nine growth stages of tomato fruit. The investigation revealed two critical links during tomato fruit development: a link between fine-tuned NAD(P) homeostasis and profound reprogramming of NAD(P) metabolism and a link between NAD(P)-dependent enzymes and central metabolic pathways, particularly those involved in mitochondrial functions (Decros et al.). These results set up the stage for more in-depth investigations of the implication of NAD(P) in fruit development.
Hence, this Research Topic benefits the scientific community by providing new insights into the many aspects of NAD metabolism and signaling. Several open questions remain in order to understand fully the regulatory circuitry of NAD metabolism and signaling.
Author Contributions
All authors wrote and/or reviewed the editorial.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Acknowledgments
The editors wish to acknowledge the reviewers who handled one or more manuscripts for this Research Topic on NAD Signaling and Metabolism in Plants. Peer review of manuscripts is often underappreciated, yet crucial to the discipline and quality of scholarship published in this journal.
References
Alferez, F. M., Gerberich, K. M., Li, J.-L., Zhang, Y., Graham, J. H., Mou, Z. (2018). Exogenous nicotinamide adenine dinucleotide induces resistance to Citrus Canker in Citrus. Front. Plant Sci. 9, 1472. doi: 10.3389/fpls.2018.01472
Berger, F., Ramírez-Hernández, M. H., Ziegler, M. (2004). The new life of a centenarian: signalling functions of NAD(P). Trends Biochem. Sci. 29, 111–118. doi: 10.1016/j.tibs.2004.01.007
Decros, G., Baldet, P., Beauvoit, B., Stevens, R., Flandin, A., Colombié, S., et al. (2019). Get the balance right: ROS homeostasis and redox signalling in fruit. Front. Plant Sci. 10, 1091. doi: 10.3389/fpls.2019.01091
Gakière, B., Fernie, A. R., Pétriacq, P. (2018a). More to NAD+ than meets the eye: a regulator of metabolic pools and gene expression in Arabidopsis. Free Radic. Biol. Med. 122, 85–96. doi: 10.1016/j.freeradbiomed.2018.01.003
Gakière, B., Hao, J., de Bont, L., Pétriacq, P., Nunes-Nesi, A., Fernie, A. R. (2018b). NAD + Biosynthesis and Signaling in Plants. Crit. Rev. Plant Sci. 37, 1–49. doi: 10.1080/07352689.2018.1505591
Hashida, S.-N., Takahashi, H., Uchimiya, H. (2009). The role of NAD biosynthesis in plant development and stress responses. Ann. Bot. 103, 819–824. doi: 10.1093/aob/mcp019
Hashida, S.-N., Miyagi, A., Nishiyama, M., Yoshida, K., Hisabori, T., Kawai-Yamada, M. (2018). Ferredoxin/thioredoxin system plays an important role in the chloroplastic NADP status of Arabidopsis. Plant J. Cell Mol. Biol. 95, 947–960. doi: 10.1111/tpj.14000
Li, B.-B., Wang, X., Tai, L., Ma, T.-T., Shalmani, A., Liu, W.-T., Liu, W.-Q., Chen, K.-M. (2018). NAD Kinases: metabolic targets controlling redox co-enzymes and reducing power partitioning in plant stress and development. Front. Plant Sci. 9, 379. doi: 10.3389/fpls.2018.00379
Pétriacq, P., de Bont, L., Hager, J., Didierlaurent, L., Mauve, C., Guérard, F., et al. (2012). Inducible NAD overproduction in Arabidopsis alters metabolic pools and gene expression correlated with increased salicylate content and resistance to Pst-AvrRpm1. Plant J. 70, 650–665. doi: 10.1111/j.1365-313X.2012.04920.x
Pétriacq, P., Ton, J., Patrit, O., Tcherkez, G., Gakière, B. (2016). NAD acts as an integral regulator of multiple defense layers. Plant Physiol. 172, 1465–1479. doi: 10.1104/pp.16.00780
Ungerer, J., Wendt, K. E., Hendry, J. I., Maranas, C. D., Pakrasi, H. B. (2018). Comparative genomics reveals the molecular determinants of rapid growth of the cyanobacterium Synechococcus elongatus UTEX 2973. Proc. Natl. Acad. Sci. 115, E11761–E11770. doi: 10.1073/pnas.1814912115
Wang, C., Zhou, M., Zhang, X., Yao, J., Zhang, Y., Mou, Z. (2017). A lectin receptor kinase as a potential sensor for extracellular nicotinamide adenine dinucleotide in Arabidopsis thaliana. eLife 6, e25474. doi: 10.7554/eLife.25474
Wang, C., Huang, X., Li, Q., Zhang, Y., Li, J.-L., Mou, Z. (2019). Extracellular pyridine nucleotides trigger plant systemic immunity through a lectin receptor kinase/BAK1 complex. Nat. Commun. 10, 4810. doi: 10.1038/s41467-019-12781-7
Keywords: plant, NAD signaling, pyridine nucleotide, NAD metabolism, fruit, cyanobacteria, light responce
Citation: Fernie A, Hashida S-n, Yoshimura K, Gakière B, Mou Z and Pétriacq P (2020) Editorial: NAD Metabolism and Signaling in Plants. Front. Plant Sci. 11:146. doi: 10.3389/fpls.2020.00146
Received: 10 December 2019; Accepted: 30 January 2020;
Published: 26 February 2020.
Edited by:
Jean Rivoal, Université de Montréal, CanadaReviewed by:
Abir U Igamberdiev, Memorial University of Newfoundland, CanadaWayne Snedden, Queen’s University, Canada
Copyright © 2020 Fernie, Hashida, Yoshimura, Gakière, Mou and Pétriacq. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Pierre Pétriacq, pierre.petriacq@inrae.fr