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SYSTEMATIC REVIEW article

Front. Cardiovasc. Med., 25 June 2025

Sec. Cardiovascular Biologics and Regenerative Medicine

Volume 12 - 2025 | https://doi.org/10.3389/fcvm.2025.1532157

Tissue response and clinical outcomes after cardiovascular use of porcine small intestinal small intestinal submucosal extracellular matrix: a systematic review


Villads Juul Bruun,&#x;Villads Juul Bruun1,†Line Lyngbak Jensen,&#x;Line Lyngbak Jensen1,†J. Michael Hasenkam,,&#x;J. Michael Hasenkam1,2,†Johannes H. Jedrzejczyk,
&#x;
Johannes H. Jedrzejczyk1,2*
  • 1Department of Cardiothoracic and Vascular Surgery, Aarhus University Hospital, Aarhus, Denmark
  • 2Department of Clinical Medicine, Aarhus University, Aarhus, Denmark

Background: Porcine small intestinal submucosal extracellular matrix (PSIS-ECM) is a biomaterial that has gained increasing popularity in cardiovascular surgery over the past three decades. This popularity is due to PSIS-ECM demonstrating properties of an ideal biological scaffold; it is easy to use, lacks immunogenicity, is absorbable, possesses the potential to promote native tissue growth, and exhibits remodelling properties. We systematically reviewed the literature on the preclinical and clinical use of this approach in cardiovascular surgery over the past decade.

Methods: Utilizing a box-search methodology, an extensive survey of the literature on PSIS-ECM's application in cardiovascular surgery from 2013 until September 2023 was conducted within the PubMed and Embase databases. Initially, 245 publications were identified. Following title, abstract, and full-text screening, 66 articles were included in the survey.

Results: Among nine preclinical studies conducting histological assessments of explants, eight did not report signs of inflammation. Tissue remodelling was documented in six preclinical studies. Histological examination of explants was incorporated into thirteen clinical cohort studies, all of which demonstrated varying intensities of inflammation and no or minimal signs of regeneration and remodeling. The reintervention rates among clinical cohort studies range from 4.5% to 87.5%. Eleven studies reported a reintervention rate exceeding 15%, while six reported a reintervention rate below 15%.

Conclusion: Preclinical studies corroborate the notion that PSIS-ECM exhibits properties of an ideal biological scaffold. However, these findings lack reproducibility in clinical settings. Combined with reports from clinical studies showing reintervention rates exceeding 15%, this has raised concerns about whether clinical application of PSIS-ECM should be confined to selected cases.

Introduction

Cardiovascular disease (CVD) is the predominant cause of global mortality (1), posing a significant challenge to global health. CVD is a heterogeneous group of diseases, including acquired and congenital pathologies. Irrespective of etiology, surgical interventions are often the primary treatment, and the procedures frequently necessitate the implantation of artificial tissue to replace or reconstruct deficient, damaged, or defective tissue. Therefore, throughout the years, several materials, encompassing autologous, xenogeneic, and synthetic materials, have been proposed to fulfill the requirements for various surgical applications. Nonetheless, these materials have both advantages and disadvantages. Biological materials, such as autologous and xenogeneic pericardium, are prone to retraction, fibrosis, thickening, and calcification (2). Synthetic materials inherently lack biocompatibility, exhibit rigidity, and promote reactive inflammation (3). Neither of these materials facilitates tissue growth, which is particularly desirable in pediatric patients. Thus, developing a material that does not exhibit these limitations has long been a sought-after goal.

Porcine small intestinal submucosal extracellular matrix (PSIS-ECM), a decellularized small intestinal submucosa, is a biological scaffold that has become increasingly popular in cardiovascular surgery over the last two decades. The popularity is based on early preclinical studies presenting results that indicate PSIS-ECM exhibits characteristics of an ideal material for cardiovascular tissue engineering. It is easy to handle, resistant to fibrosis and calcification, lacks immunogenicity, and possesses the potential to promote infiltration of host cells and tissue growth.

Since the first clinical application of PSIS-ECM in 2010, it has been utilized for various purposes in cardiovascular surgery. Nezhad et al. have published a comprehensive literature review on the utilization of PSIS-ECM up to 2015 (3). However, as PSIS-ECM had only been in clinical use for five years at the time of their study, the number of included clinical cohort studies in their review is limited. Consequently, we conducted a similar study focusing on more recently published research. Here, we present the results of preclinical and clinical studies on the application of PSIS-ECM over the last decade in both adult and pediatric patients.

Methods

Utilizing a box-search methodology, an extensive literature survey was conducted from 2013 to September 2023, encompassing the PubMed and Embase databases. The search string employed was as follows: [“CorMatrix” OR “SIS-ECM” OR “Small Intestine Submucosa Extracellular Matrix” OR “Small Intestinal Submucosa Extracellular Matrix”] AND [“Cardiovascular Surgery” OR “Heart Surgery” OR “Cardiothoracic Surgery” OR “Cardiovascular Disease/surgery* (MeSH)]”. Figure 1 illustrates a schematic representation of the literature search process, following the PRISMA 2020 flow chart. Two independent authors screened the articles (VJB and LLJ). Only articles chosen by both authors at each screening step proceeded to the next stage. Just the abstracts of articles with either relevant titles or keywords were assessed. The complete content of potentially relevant articles was examined for potential inclusion in the study, resulting in the inclusion and tabulation of 66 articles (Tables 13). Any discrepancies in the inclusion of articles were identified and resolved via discussion. The scientific methodological quality of the included articles was evaluated. However, no quantitative rating tool was employed because “quality assessment of observational studies is not typically conducted in systematic reviews” (4).

Figure 1
Flowchart detailing study selection process. Top section lists inclusion criteria (published after 2012, English, in vivo) and exclusion criteria (before 2013, in vitro, reviews). Search string used includes terms like

Figure 1. PRISMA-style flow chart of the study selection process. The literature search identified 245 records through PubMed and Embase. After removing 47 duplicates, 198 studies were screened by title, of which 76 were excluded. Of the 122 abstracts screened, 22 were excluded. Following a full-text screening of 100 articles, 34 were excluded due to the unavailability of full text (n = 18), reviews (n = 10), the use of acute models (n = 5), or the lack of reported results (n = 1). Ultimately, 66 studies were included: 11 preclinical (in vivo) studies and 55 clinical studies, further classified as case studies (n = 28) or cohort studies (n = 27).

Table 1
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Table 1. Preclinical studies on the utilization porcine small intestinal submucosal extracellular matrix.

Table 2
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Table 2. Clinical case studies on the utilization of porcine small intestinal submucosal extracellular matrix.

Table 3
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Table 3. Clinical cohort studies on the utilization of porcine small intestinal submucosal extracellular matrix.

Results

Preclinical trials evaluating the outcomes of utilizing PSIS-ECM in cardiovascular surgery have been conducted since the early 2000s (3). Over the past decade, PSIS-ECM has been employed in experimental cardiovascular surgery involving various animal models, including mice (5, 6), rats (7), sheep, lambs (812), and pigs (1315). The application of PSIS-ECM has been diverse, including tricuspid valve replacement (8, 9), pulmonary artery replacement (12, 14), and myocardial repair post-infarction (57, 10, 11, 13, 15). The results of these studies are presented in Table 1. A recent strategy involves augmenting PSIS-ECM by incorporating growth factors or cardiac cells (57, 13). The results of these studies are also shown in Table 1.

Overall, the findings of the preclinical studies corroborate the notion that PSIS-ECM exhibits minimal immunogenicity. Among the nine studies that entailed histological assessments of explants, eight reported no signs of inflammation or inflammatory reactions (5, 711, 13, 14). The singular exception was the study by Van Rijswijk et al. (12), where chronic inflammation and fibrosis were noted. Tissue remodelling, which involves the infiltration of various cells, such as fibroblasts, smooth muscle cells, adipocytes, and endothelial cells, along with the processes of neovascularization and reendothelialization, has been documented in six studies (810, 13, 14). However, Van Rijswijk et al. merely observed limited indications of tissue remodelling. In myocardial repair, the use of PSIS-ECM patches appears to enhance regeneration, thereby improving post-infarction cardiac function (57, 10, 11, 13, 15). This effect is particularly notable when PSIS-ECM is infused with either growth factors or cardiac cells (57, 13).

Since its initial clinical application, PSIS-ECM has found extensive use across a broad spectrum of cases, including pericardial reconstruction (16), valve repair or replacement (1720), diverse congenital heart defects (1719, 2127), vascular repair (2835), myocardial repair (3639), and endocarditis (29, 4043). The utilization of PSIS-ECM has extended to both adult and pediatric patients. Table 2 presents the outcomes of case studies conducted within the past decade.

The case study results indicate that pediatric patients experience less favorable outcomes and a higher rate of reintervention compared to adults. Of the nine pediatric cases reviewed, five (55%) presented significant complications, such as vessel stenosis, valve regurgitation, or atrial and ventricular dilatation, all of which required additional surgical or interventional cardiology procedures (18, 19, 22, 23, 25). In contrast, adult cases demonstrated fewer complications, with only four out of 19 cases (21%) reporting issues such as regurgitation, pseudoaneurysm formation, patch dehiscence, or early patch failure necessitating reoperation (27, 32, 37, 42).

Two specific case studies (25, 37) raised concerns about the durability of the PSIS-ECM patch. In one case, Mously et al. reported that a PSIS-ECM patch used for ventricular septal defect repair in a 58-year-old woman following myocardial infarction failed within seven months due to nearly complete reabsorption. Similarly, Rodrigues et al. observed almost total disintegration of the PSIS-ECM patch within just one month post-surgery in a 5-year-old patient with Tetralogy of Fallot and hypoplastic pulmonary arteries. These findings highlight potential weaknesses in using PSIS-ECM patches, particularly in pediatric patients, where rapid degeneration may lead to early failure.

Explants were available for histological analysis in 12 case studies (1619, 23, 25, 26, 30, 32, 33, 42, 43). Among these, three case studies (16, 30, 33) documented a positive remodelling process with minimal or no signs of inflammation. Conversely, nine case studies (1719, 23, 25, 26, 32, 42, 43) reported mild to severe chronic inflammation with no or minimal signs of remodelling. Additionally, Nezhad et al. observed calcification of the explant. Abu Saleh et al. compared a PSIS-ECM explant, which had been in vivo for 14 months, to an unused PSIS-ECM patch, revealing nearly identical cellularity and organization.

Table 3 presents the results of clinical cohort studies using PSIS-ECM in cardiovascular surgery over the last decade. The majority of these studies were retrospective in design (4467), corresponding to evidence level III, with a primary focus on pediatric populations. Only three studies were conducted prospectively, two involving pediatric populations and one involving adult populations (6870), placing them at evidence level II.

As presented in Table 3, PSIS-ECM has been employed for diverse purposes in clinical settings. These include various congenital heart defects (45, 46, 4953, 55, 5763, 6669), vascular repair/reconstruction (54, 62, 64, 70), valve repair/replacement (47, 48, 56, 60, 61, 65), and complications arising from myocardial infarction (44).

Of the 27 clinical cohort studies, 13 (45, 46, 49, 5153, 56, 5860, 63, 68, 69) incorporated histological examination of explants, all of which demonstrated inflammation. The intensity and type of inflammation displayed varied among the studies, with the majority (45, 46, 49, 5153, 56, 63) indicating chronic inflammation. This was characterized by inflammatory cell types, including macrophages, lymphocytes, eosinophilic cells, and giant cells. Furthermore, several studies have shown calcification (5153, 60, 69) of the PSIS-ECM patch. No, or minimal signs of regeneration or remodelling were observed in the 13 studies.

A common outcome assessed across many of the studies is the reintervention rate. While specific studies report a relatively low reintervention rate of 4.5% (64), others report a significantly higher rate of 87.5% (61). Four studies (56, 61, 62, 66) are particularly noteworthy, given that they are comparative studies in which PSIS-ECM patches were compared to autologous pericardium patches.

In the study by Hu et al., 87.5% (7 out of 8) of their patients (mean age 13.63 ± 6.5 years) who underwent aortic valve repair with PSIS-ECM necessitated reoperation due to severe aortic regurgitation. In contrast, only 37.5% (11 out of 30) of the patients (mean age 10.25 ± 6.56 years) undergoing the same procedure with autologous pericardium required reoperation due to moderate/severe aortic regurgitation (61). The follow-up period for this study was five years.

Kelley et al. observed that 32% (8 out of 25) of their patients who underwent anterior leaflet augmentation of the mitral valve using PSIS-ECM required reoperation due to severe regurgitation. Conversely, none of the patients undergoing the same procedure with autologous pericardium required reoperation (56). The mean age in both groups was 62.6 ± 12.2 years, and the follow-up for this study extended up to 12 months.

Ebert et al. presented that 16.2% (6 out of 37) of their patients who underwent pulmonary artery reconstruction with PSIS-ECM needed reintervention. However, only 7.3% (3 out of 41) required reintervention among the patients undergoing the same procedure utilizing autologous pericardium. The median age in both groups was 12.1 months (5.6–59.5) (62).

Weis et al. noted that 55.5% (10 out of 18) of patients who underwent corrective repair of Tetralogy of Fallot or an Arterial Switch Procedure with PSIS-ECM required reoperation. Of these, 70% (7 out of 10) were attributed to patch failure. In comparison, 30.6% (11 out of 30) of the patients undergoing the same procedure with autologous pericardium required reoperation. Of these 11 patients, 27.3% (3 out of 11) were attributed to patch failure (66). The follow-up periods were 47 months for patients treated with PSIS-ECM and 121 months for patients treated with autologous pericardium. The median age of the study population was 11 months (2–60).

Among the case studies, less favorable outcomes and higher reintervention rates were evident in pediatric cases. Interestingly, this trend was not apparent in the clinical cohort studies. In 18 out of 27 clinical cohort studies, the reintervention rate was used as an outcome measure; five investigated adult patient populations, and thirteen studied pediatric patient populations. Three out of five (60%) (48, 54, 56) clinical cohort studies investigating adult patient populations reported reintervention rates exceeding 15%. Comparably, in nine out of thirteen (69.2%) (49, 50, 53, 6062, 66, 68, 69) clinical cohort studies, pediatric patient populations presented reintervention rates greater than 15%.

Discussion

Since the early 2000s, preclinical studies on the application of PSIS-ECM in experimental cardiovascular surgery have been conducted. Preliminary findings showed substantial promise, indicating that this biomaterial possessed characteristics indicative of an ideal material for cardiovascular tissue repair and cardiovascular tissue engineering. The material exhibited remodelling properties, absorbability, lack of immunogenicity, and potential to promote native tissue growth (3). These findings ultimately led to the first clinical application of PSIS-ECM in 2010. Since then, it has been applied in numerous clinical applications within cardiovascular surgery.

Manufacturers of CorMatrix claim that PSIS-ECM is a bioscaffold that facilitates native tissue growth and remodeling. This notion was upheld by early preclinical studies documenting remodelling, infiltration of host cells, endothelization, and neoangiogenesis (3). Recent preclinical studies conducted over the past decade have further corroborated this claim. Across all preclinical investigations that performed a histological assessment of explants featured in this review, the histological examination consistently indicated tissue remodeling with host cell infiltration of mesenchymal cells, adipocytes, fibroblasts, endothelial cells, and cardiomyocytes. Additionally, the studies disclosed neoangiogenesis, endothelialization, and tissue organization similar to native tissue (5, 711, 13, 14).

Contrary to expectations, these findings have scarcely been reproduced in clinical settings. Thirteen clinical cohort studies conducting histological analysis of explants were reviewed. Among these, twelve studies reported minimal or no infiltration of host cells and found no evidence of remodeling in the explants to resemble native tissue (18, 45, 46, 49, 5153, 56, 59, 60, 63, 68). Cox et al. stand as the sole exception, presenting evidence of neo-intima, endothelialization, and the infiltration of smooth muscle cells into the graft (58).

The differences in tissue remodelling between animal models and humans are poorly understood, particularly in PSIS-ECM scaffold studies. One possible explanation is the scaffold's xenogeneic nature, which may provoke different responses in human patients. However, the remodeling process has been consistently observed in xenogeneic animal models, such as sheep, rats, and mice (5, 711), suggesting that additional factors may contribute to the discrepancy.

For example, preclinical studies typically use healthy animals with normal physiology, while patients in clinical studies often have significant underlying pathologies. Since variations between healthy and pathological conditions are likely to influence tissue regeneration, it is essential to investigate whether the positive outcomes seen in preclinical models can be replicated in pathological animal models. Such studies could provide more translational and accurate insights into how PSIS-ECM scaffolds perform in clinical scenarios.

Despite preclinical studies demonstrating that PSIS-ECM exhibits properties associated with tissue remodelling and the potential for native tissue growth, the prospects of complete reabsorption and replacement with native tissue in humans are questionable. The skepticism arises from the fact that 12 out of 13 clinical studies in the current review report either no or minimal infiltration of host cells and no tissue remodelling when PSIS-ECM is employed in humans.

The outcomes presented in this review imply a distinct variation in the immunogenic response to PSIS-ECM between animal subjects and humans. Out of nine preclinical studies incorporating histological assessment of explants, eight reported no signs of inflammation or inflammatory reaction (5, 711, 13, 14). Contrarily, all 13 clinical studies that conducted histological analysis of explants demonstrated inflammation of varying intensity, with the majority displaying chronic inflammation (45, 46, 49, 5153, 56, 5860, 63, 68, 69). Similar findings have been reported by Nezhad et al. (3).

PSIS-ECM has undergone decellularization, lowering the risk of triggering the human immune system. Nonetheless, PSIS-ECM is xenogeneic when employed in humans, thus potentially expressing epitopes that can activate immunological pathways and induce inflammatory reactions. Interestingly, several preclinical studies using PSIS-ECM in various xenogeneic animal models, such as sheep (812, 15, 7174), rats (7, 75), mice (5, 6), and calves (76, 77), yielded results without signs of inflammation. Only three preclinical studies utilizing PSIS-ECM in a xenogeneic model since the 2000s have identified inflammation upon histological examination of explants (12, 78, 79).

The difference in the immunogenic response to PSIS-ECM between animal subjects and humans requires further investigation. Rosario-Quinones et al. suggest that the variance in immunogenicity may stem from PSIS-ECM expressing gal-a epitopes, to which humans possess natural antibodies (49). However, this is only speculation; further inquiry is warranted to amplify our knowledge.

We found a considerable variance in the reported reintervention rates among the clinical cohort studies included in the present review. Reintervention rates may be influenced by a variety of factors, including the expertise and proficiency of the surgeon conducting the procedure, the characteristics of the patient population, the anatomical site where the PSIS-ECM patch is applied, the immunological response to the PSIS-ECM patch, the number of layers of the PSIS-ECM patch, and the duration of the follow-up period.

The anatomical site of PSIS-ECM patch implantation appears to be particularly important. Padalino et al. concluded that PSIS-ECM is characterized by good late outcomes for septal and aortic wall reconstruction, but suboptimal functional late performance when used to reconstruct semilunar valve leaflets or small pulmonary arteries (68). Weis et al. reached a similar conclusion, declaring that PSIS-ECM performed well for the closure of atrial and ventricular defects. In contrast, using PSIS-ECM for arterial vessel reconstruction and valve reconstruction was associated with higher reintervention rates compared to standard autologous pericardium (66). Witt et al. correspondingly noted that PSIS-ECM performed well in pediatric cardiovascular reconstructions, particularly those with septal defects or vascular patch augmentation remote from the outflow tract (45). Consistent with these observations, three out of five clinical cohort studies (55, 57, 64) reporting reintervention rates below 15% utilized PSIS-ECM for septal defects or extracardiac vessel repair. Witt et al. and Haney et al., who also report a reintervention rate of less than 15%, applied PSIS-ECM at various sites, including valves, the interventricular septum, and extracardiac vessels. However, the majority of the sites were septal or extracardiac vessels. The clinical studies in this review reporting reintervention rates greater than 15% primarily employed PSIS-ECM for valve reconstruction, valve replacement, or pulmonary artery repair (4850, 53, 56, 6062, 66, 68, 69). The available evidence suggests that PSIS-ECM performs more reliably in low-pressure, extracardiac or septal settings. In contrast, applications involving valve or outflow tract reconstructions are associated with higher rates of reintervention. However, due to limited data granularity, inconsistent reporting, and the absence of prospective stratification, further generalization is not currently supported.

The disparity in reintervention rates across various anatomical locations may be related to variations in hemodynamic pressure exerted on the patches. Hemodynamic pressures and the mechanical stresses they impose on tissues vary among anatomical sites, and it is established that mechanical stress influences the regeneration processes (80). Thus, the hemodynamic conditions near the interventricular septum could promote regeneration more efficiently than those near the valve locations and the pulmonary artery site. Despite this, the precise relationship between the positioning of the PSIS-ECM patch and the reintervention rate is yet to be fully understood.

As previously addressed, all included clinical cohort studies assessing the histology of explants document varying degrees of inflammation. It remains to be determined whether the inflammatory response resulting from the implantation of PSIS-ECM patches contributes to the deterioration and eventual malfunction, thereby necessitating reintervention. A paradox arises in this context. Inflammation is an inherent aspect of the recellularization process, and it remains unclear whether the observed inflammation would have contributed to regeneration had the follow-up period been extended. However, this cannot be determined, as patch failure prevents the possibility of prolonging the follow-up period.

Another potential factor contributing to the variability in clinical and histological outcomes is the heterogeneity in the source and preparation method of the PSIS-ECM scaffolds. While some studies explicitly stated that they used commercially available products, such as CorMatrix®, others did not specify whether the scaffold was commercially sourced or produced in-house. Moreover, very few studies provided technical details about the decellularization protocols or batch-specific characteristics. This lack of standardization complicates the interpretation of outcomes across studies and may partially account for the discrepancies in inflammation, remodeling, and rates of reintervention. Future studies should report the source and preparation details of the scaffold more rigorously to enable better reproducibility and cross-study comparison.

The number of layers in PSIS-ECM might affect the surgical outcomes. It is speculated that the risk of thrombosis between the layers, leading to patch failure and the need for reintervention, increases with the number of layers in the patch. Furthermore, the mechanical stresses exerted on the patches are contingent upon the number of layers in the patch. As mechanical stress is known to influence the regeneration process, the number of layers could potentially affect the regeneration process and, therefore, the reintervention rate.

Haney et al. are the sole researchers to have conducted a clinical comparative analysis of the outcomes of utilizing 2-ply vs. 4-ply PSIS-ECM in cardiovascular surgery (67). Surgical reintervention was required in 2.1% of patients treated with 4-ply PSIS-ECM, while 6.3% of patients treated with 2-ply PSIS-ECM underwent surgical reintervention. Percutaneous intervention was performed on 6.4% of patients treated with 4-ply PSIS-ECM. In comparison, 9.4% of patients treated with 2-ply PSIS-ECM necessitated percutaneous intervention. However, the sample size was considerably larger for 4-ply PSIS-ECM (n = 372) compared to 2-ply PSIS-ECM (n = 32). Additionally, conclusions should not be drawn from a single-center study. Accordingly, further research is required to unfold the impact of various layers in the PSIS-ECM patches.

As noted in the results section, case studies demonstrated less favorable outcomes in pediatric patients than in adults. However, this trend was not apparent in the clinical cohort studies. Given that clinical cohort studies provide stronger evidence than case studies, we do not consider the correlation between age and outcomes significant.

The source of the high reintervention rate is likely multifactorial, making it challenging to determine the precise causative factors. Nonetheless, it is worrisome that most clinical studies in this review reported a reintervention rate greater than 15%. Based on their findings from clinical cohort studies reporting reintervention rates exceeding 10%, Nezhad et al. raised ethical concerns about conducting more extensive clinical studies using PSIS-ECM in the pediatric population (3). Thus, our findings further emphasize these concerns articulated by Nezhad et al.

As indicated in the results section, only three studies included in this review are classified as evidence level II, with the remainder falling under evidence levels III or IV. While this may not pose a significant issue due to the number of included studies with similar findings, the overall strength of the evidence would have been greater if the majority of studies had been at evidence level II.

A limited number of comparative studies have been conducted to date, comparing PSIS-ECM to well-established materials, such as autologous pericardium, xenogeneic pericardium, and synthetic materials (56, 61, 62, 66, 81). However, these studies were undertaken retrospectively. Thus, additional, preferably prospective and comparative, research is required. Furthermore, the significance of the layer count of the PSIS-ECM material required further evaluation. Investigating the vacuum-pressed iteration of PSIS-ECM by CorMatrix would also be of interest. Considering the high reintervention rate in many clinical cohort studies reviewed in this present review, conducting these studies in a xenogeneic animal model should precede the transition to a clinical setting, preferably a pathological animal model.

Preclinical studies (57, 10, 11, 13, 15) and clinical case studies (36, 38) have shown that applying a PSIS-ECM patch to cover a damaged area post-acute myocardial infarction can improve heart regeneration. Therefore, further preclinical research and clinical cohort studies on this topic are warranted. Additionally, preclinical trials indicate that this effect is enhanced when the PSIS-ECM is augmented with growth factors or cardiac cells (57, 13). However, limited knowledge exists, necessitating further research on the impact of augmenting PSIS-ECM with diverse growth factors and cells.

Limitations

Our literature search was confined to two databases, which could have excluded relevant studies. Additionally, there is great variability in the indications and outcome measures across the studies included in this review, which may lead to less generalizable conclusions. Furthermore, many studies did not specify whether the PSIS-ECM used was commercially available or laboratory-prepared, nor did they provide details on the decellularization protocol or characterization of the scaffold. This variability in scaffold origin and preparation introduced additional heterogeneity, which could have influenced the observed histological and clinical outcomes.

Conclusion

Preclinical studies over the past decade have consistently demonstrated that PSIS-ECM possesses several attributes of an ideal biological scaffold. However, these theoretical advantages have yet to be replicated in clinical applications. Clinical studies have reported significant limitations, including inflammation, calcification, a lack of remodelling, and minimal host cell infiltration. Furthermore, reintervention rates exceeding 15% in many clinical cohorts highlight concerns regarding the long-term durability of PSIS-ECM in cardiovascular surgery.

Despite these challenges, the selective application of PSIS-ECM, such as in septal defects or extracardiac vascular pathologies, has shown comparatively better outcomes with low reintervention rates. These findings suggest that its clinical use should be restricted to specific anatomical sites where the risk-benefit profile is more favourable.

The disparity between preclinical and clinical outcomes underscores the need for further investigation. Future research should prioritize understanding the mechanisms underlying these discrepancies, with a main focus on inflammation, calcification, and remodelling. To address these questions, we propose using pathological xenogeneic animal models as a bridge to improve translational insights before advancing to additional clinical studies. Such an approach may enhance the predictive value of preclinical findings and inform the safe and practical application of PSIS-ECM in cardiovascular repair.

Data availability statement

The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author.

Author contributions

VB: Methodology, Writing – original draft, Writing – review & editing. LJ: Writing – review & editing, Methodology. JH: Project administration, Resources, Supervision, Writing – review & editing. JJ: Conceptualization, Supervision, Writing – review & editing.

Funding

The author(s) declare that financial support was received for the research and/or publication of this article. This work was supported by the Novo Nordisk Foundation [Grant number NNF20OC0065584].

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Generative AI statement

The author(s) declare that no Generative AI was used in the creation of this manuscript.

Publisher's note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Abbreviations

CVD, cardiovascular disease; PSIS-ECM, porcine small intestinal submucosa extracellular matrix.

References

1. (WHO). W.H.O. The top 10 causes of death. (2020). Available at: https://www.who.int/news-room/fact-sheets/detail/the-top-10-causes-of-death. (Accessed October 24, 2024).

Google Scholar

2. De Martino A, Milano AD, Bortolotti U. Use of pericardium for cardiac reconstruction procedures in acquired heart diseases-A comprehensive review. Thorac Cardiovasc Surg. (2021) 69:83–91. doi: 10.1055/s-0039-1697918

PubMed Abstract | Crossref Full Text | Google Scholar

3. Mosala Nezhad Z, Poncelet A, de Kerchove L, Gianello P, Fervaille C, El Khoury G. Small intestinal submucosa extracellular matrix (CorMatrix®) in cardiovascular surgery: a systematic review. Interact Cardiovasc Thorac Surg. (2016) 22:839–50. doi: 10.1093/icvts/ivw020

PubMed Abstract | Crossref Full Text | Google Scholar

4. Mallen C, Peat G, Croft P. Quality assessment of observational studies is not commonplace in systematic reviews. J Clin Epidemiol. (2006) 59:765–9. doi: 10.1016/j.jclinepi.2005.12.010

PubMed Abstract | Crossref Full Text | Google Scholar

5. Toeg HD, Tiwari-Pandey R, Seymour R, Ahmadi A, Crowe S, Vulesevic B, et al. Injectable small intestine submucosal extracellular matrix in an acute myocardial infarction model. Ann Thorac Surg. (2013) 96:1686–94; discussion 1694. doi: 10.1016/j.athoracsur.2013.06.063

PubMed Abstract | Crossref Full Text | Google Scholar

6. Wang H, Sridhar B, Leinwand LA, Anseth KS. Characterization of cell subpopulations expressing progenitor cell markers in porcine cardiac valves. PLoS One. (2013) 8:e69667. doi: 10.1371/journal.pone.0069667

PubMed Abstract | Crossref Full Text | Google Scholar

7. Mewhort HEM, Turnbull JD, Meijndert HC, Ngu JMC, Fedak PWM. Epicardial infarct repair with basic fibroblast growth factor-enhanced CorMatrix-ECM biomaterial attenuates postischemic cardiac remodeling. J Thorac Cardiovasc Surg. (2014) 147:1650–9. doi: 10.1016/j.jtcvs.2013.08.005

PubMed Abstract | Crossref Full Text | Google Scholar

8. Fallon AM, Goodchild TT, Cox JL, Matheny RG. In vivo remodeling potential of a novel bioprosthetic tricuspid valve in an ovine model. J Thorac Cardiovasc Surg. (2014) 148:333–40.e1. doi: 10.1016/j.jtcvs.2013.10.048

PubMed Abstract | Crossref Full Text | Google Scholar

9. Zafar F, Hinton RB, Moore RA, Baker RS, Bryant R, Narmoneva DA, et al. Physiological growth, remodeling potential, and preserved function of a novel bioprosthetic tricuspid valve. J Am Coll Cardiol. (2015) 66:877–88. doi: 10.1016/j.jacc.2015.06.1091

PubMed Abstract | Crossref Full Text | Google Scholar

10. Scully BB, Fan C, Grigoryan B, Jacot JG, Vick GW, Kim JJ, et al. Remodeling of ECM patch into functional myocardium in an ovine model: a pilot study. J Biomed Mater Res B Appl Biomater. (2016) 104:1713–20. doi: 10.1002/jbm.b.33484

PubMed Abstract | Crossref Full Text | Google Scholar

11. Baker RS, Zafar F, Kimura N, Knilans T, Osinska H, Robbins J, et al. In vivo remodeling of an extracellular matrix cardiac patch in an ovine model. ASAIO J. (2019) 65:744–52. doi: 10.1097/MAT.0000000000000864

PubMed Abstract | Crossref Full Text | Google Scholar

12. van Rijswijk JW, Talacua H, Mulder K, van Hout GPJ, Bouten CVC, Gründeman PF, et al. Failure of decellularized porcine small intestinal submucosa as a heart valved conduit. J Thorac Cardiovasc Surg. (2020) 160:e201–15. doi: 10.1016/j.jtcvs.2019.09.164

PubMed Abstract | Crossref Full Text | Google Scholar

13. Tanaka A, Kawaji K, Patel AR, Tabata Y, Burke MC, Gupta MP, et al. In situ constructive myocardial remodeling of extracellular matrix patch enhanced with controlled growth factor release. J Thorac Cardiovasc Surg. (2015) 150:1280–90.e2. doi: 10.1016/j.jtcvs.2015.07.073

PubMed Abstract | Crossref Full Text | Google Scholar

14. Miller JR, Henn MC, Lancaster TS, Lawrance CP, Schuessler RB, Shepard M, et al. Pulmonary valve replacement with small intestine submucosa-extracellular matrix in a porcine model. World J Pediatr Congenit Heart Surg. (2016) 7:475–83. doi: 10.1177/2150135116651113

PubMed Abstract | Crossref Full Text | Google Scholar

15. Mewhort HEM, Turnbull JD, Satriano A, Chow K, Flewitt JA, Andrei A-C, et al. Epicardial infarct repair with bioinductive extracellular matrix promotes vasculogenesis and myocardial recovery. J Heart Lung Transplant. (2016) 35:661–70. doi: 10.1016/j.healun.2016.01.012

PubMed Abstract | Crossref Full Text | Google Scholar

16. Stelly M, Stelly TC. Histology of CorMatrix bioscaffold 5 years after pericardial closure. Ann Thorac Surg. (2013) 96:e127–129. doi: 10.1016/j.athoracsur.2013.06.114

PubMed Abstract | Crossref Full Text | Google Scholar

17. Bibevski S, Scholl FG. Feasibility and early effectiveness of a custom, hand-made systemic atrioventricular valve using porcine extracellular matrix (CorMatrix) in a 4-month-old infant. Ann Thorac Surg. (2015) 99:710–2. doi: 10.1016/j.athoracsur.2014.04.140

PubMed Abstract | Crossref Full Text | Google Scholar

18. Guariento A, Burke R, Fedrigo M, Angelini A, Maschietto N, Vida V, et al. Novel valve replacement with an extracellular matrix scaffold in an infant with single ventricle physiology. Cardiovasc Pathol. (2016) 25:165–8. doi: 10.1016/j.carpath.2015.09.003

PubMed Abstract | Crossref Full Text | Google Scholar

19. Mosala Nezhad Z, Baldin P, Poncelet A, El Khoury G. Calcific degeneration of CorMatrix 4 years after bicuspidization of unicuspid aortic valve. Ann Thorac Surg. (2017) 104:e431–3. doi: 10.1016/j.athoracsur.2017.07.040

PubMed Abstract | Crossref Full Text | Google Scholar

20. Szałański P, Uziębło-Życzkowska B, Zaleska M. Combined total mitral and tricuspid valve reconstruction with the use of CorMatrix in an adult. Interact Cardiovasc Thorac Surg. (2019) 28:158–60. doi: 10.1093/icvts/ivy200

Crossref Full Text | Google Scholar

21. Eckhauser AW, Hannon D, Molitor M, Scaife E, Gruber PJ. Repair of traumatic aortoinnominate disruption using CorMatrix. Ann Thorac Surg. (2013) 95:e99–e101. doi: 10.1016/j.athoracsur.2012.09.060

PubMed Abstract | Crossref Full Text | Google Scholar

22. Deorsola L, Pace Napoleone C, Abbruzzese PA. Repair of an unusual aortic coarctation using an extracellular matrix patch. Ann Thorac Surg. (2014) 97:1059–61. doi: 10.1016/j.athoracsur.2013.06.109

PubMed Abstract | Crossref Full Text | Google Scholar

23. Fernández-Doblas J, Palmer NE, Pérez-Andreu J, Reyes-Juárez JL, Albert DC, Abella R. Correction of congenital cardiac defects with CorMatrix extracellular matrix in pediatric patients: is it really safe? Rev Espanola Cardiol Engl Ed. (2016) 69:787–9. doi: 10.1016/j.rec.2016.03.020

PubMed Abstract | Crossref Full Text | Google Scholar

24. Khan MS, Yeager MR, Bryant R, Lorts A, Morales DLS. Coronary artery reconstruction using a bioengineered patch and epicardial tunnel. Ann Thorac Surg. (2016) 101:363–5. doi: 10.1016/j.athoracsur.2015.03.072

PubMed Abstract | Crossref Full Text | Google Scholar

25. Rodrigues C, Cerejo R, Rodrigues R, Pinto E, Fragata J. Right ventricular outflow tract pseudo aneurysm after reconstruction with small intestinal submucosal cormatrix patch A word of caution. Rev Port Cir Cardiotorac Vasc. (2020) 27:117–9. doi: 10.48729/pjctvs.59

PubMed Abstract | Crossref Full Text | Google Scholar

26. Bibevski S, Ruzmetov M, Ladich E, Mendoza LE, Scholl FG. Reconstruction of the neopulmonary root after coronary button harvest for arterial switch operation using 2-ply extracellular matrix (tyke): a post-implant histology. Front Cardiovasc Med. (2020) 7:562136. doi: 10.3389/fcvm.2020.562136

PubMed Abstract | Crossref Full Text | Google Scholar

27. Burke CR, Lewis E, Schreiter NA, Bartlett H, Krieger EV, Anagnostopoulos PV, et al. Is porcine small intestinal submucosal extracellular matrix (ECM) a suitable material for right ventricular outflow tract reconstruction in association with pulmonary valve replacement? World J Pediatr Congenit Heart Surg. (2022) 13:518–21. doi: 10.1177/21501351211069559

PubMed Abstract | Crossref Full Text | Google Scholar

28. Yeen WC, Faber C, Caldeira C, Nallamshetty L, Haddad T, Rolfe M. Reconstruction of pulmonary venous conduit with CorMatrix in lung transplant. Asian Cardiovasc Thorac Ann. (2013) 21:360–2. doi: 10.1177/0218492312454728

PubMed Abstract | Crossref Full Text | Google Scholar

29. Madden NJ, Troutman DA, DeMarsico AJ. Use of a small intestine submucosa extracellular matrix patch in repeated carotid endarterectomy. J Am Osteopath Assoc. (2014) 114:732–4. doi: 10.7556/jaoa.2014.143

PubMed Abstract | Crossref Full Text | Google Scholar

30. Slachman FN. Constructive remodeling of CorMatrix extracellular matrix after aortic root repair in a 90-year-old woman. Ann Thorac Surg. (2014) 97:e129–131. doi: 10.1016/j.athoracsur.2013.10.103

PubMed Abstract | Crossref Full Text | Google Scholar

31. Szczeklik M, Gupta P, Amersey R, Lall KS. Reconstruction of the right atrium and superior vena cava with extracellular matrix. J Card Surg. (2015) 30:351–4. doi: 10.1111/jocs.12398

PubMed Abstract | Crossref Full Text | Google Scholar

32. Abu Saleh WK, Al Jabbari O, Grande-Allen J, Ramchandani M. Extracellular matrix scaffold as a tubular graft for ascending aorta aneurysm repair. J Card Surg. (2015) 30:648–50. doi: 10.1111/jocs.12583

PubMed Abstract | Crossref Full Text | Google Scholar

33. Ferng A, Connell A, Nunez M, Johnson K, Braunhut B, Lick S, et al. Cardiac regeneration in the human left ventricle after CorMatrix implantation. Ann Thorac Surg. (2017) 104:e239–41. doi: 10.1016/j.athoracsur.2017.03.044

PubMed Abstract | Crossref Full Text | Google Scholar

34. Ali JM, Dunning J. Stented CorMatrix® conduit to bypass benign superior vena caval obstruction. Eur J Cardiothorac Surg. (2018) 53:472–4. doi: 10.1093/ejcts/ezx303

PubMed Abstract | Crossref Full Text | Google Scholar

35. Brinster DR, Patel JA. The use of CorMatrix extracellular matrix for aortic root enlargement. J Cardiothorac Surg. (2014) 9:178. doi: 10.1186/s13019-014-0178-5

PubMed Abstract | Crossref Full Text | Google Scholar

36. Bhatt A, Bates MJ, Marcu CB, Matheny RG, Carabello BA, Yin K, et al. Second-generation extracellular matrix patch for epicardial infarct repair. J Cardiothorac Surg. (2023) 18:255. doi: 10.1186/s13019-023-02358-3

PubMed Abstract | Crossref Full Text | Google Scholar

37. Mously H, Kim J, Wheat HL, Sayed A, Elgudin Y. Recurrent ventricular septal defect following closure CorMatrix: a case report. J Card Surg. (2021) 36:392–5. doi: 10.1111/jocs.15163

PubMed Abstract | Crossref Full Text | Google Scholar

38. Holubec T, Caliskan E, Bettex D, Maisano F. Repair of post-infarction left ventricular free wall rupture using an extracellular matrix patch. Eur J Cardiothorac Surg. (2015) 48:800–3. doi: 10.1093/ejcts/ezu521

PubMed Abstract | Crossref Full Text | Google Scholar

39. Yanagawa B, Rao V, Yau TM, Cusimano RJ. Potential myocardial regeneration with CorMatrix ECM: a case report. J Thorac Cardiovasc Surg. (2014) 147:e41–43. doi: 10.1016/j.jtcvs.2013.12.012

PubMed Abstract | Crossref Full Text | Google Scholar

40. Sündermann SH, Rodriguez Cetina Biefer H, Emmert MY, Falk V. Use of extracellular matrix materials in patients with endocarditis. Thorac Cardiovasc Surg. (2014) 62:76–9. doi: 10.1055/s-0032-1328920

PubMed Abstract | Crossref Full Text | Google Scholar

41. Wallen J, Rao V. Extensive tricuspid valve repair after endocarditis using CorMatrix extracellular matrix. Ann Thorac Surg. (2014) 97:1048–50. doi: 10.1016/j.athoracsur.2013.05.117

PubMed Abstract | Crossref Full Text | Google Scholar

42. Toyoda Y, Kashem MA, Hisamoto K, Shiose A. Tricuspid valve reconstruction with the extracellular matrix tube technique: a word of caution. J Thorac Cardiovasc Surg. (2014) 148:e141–143. doi: 10.1016/j.jtcvs.2014.04.015

PubMed Abstract | Crossref Full Text | Google Scholar

43. Luk A, Rao V, Cusimano RJ, David TE, Butany J. Cormatrix extracellular matrix used for valve repair in the adult: is there de novo valvular tissue seen? Ann Thorac Surg. (2015) 99:2205–7. doi: 10.1016/j.athoracsur.2014.08.063

PubMed Abstract | Crossref Full Text | Google Scholar

44. Yanagawa B, Rao V, Yau TM, Cusimano RJ. Initial experience with intraventricular repair using CorMatrix extracellular matrix. Innovations (Phila). (2013) 8:348–52. doi: 10.1097/IMI.0000000000000014

PubMed Abstract | Crossref Full Text | Google Scholar

45. Witt RG, Raff G, Van Gundy J, Rodgers-Ohlau M, Si M-S. Short-term experience of porcine small intestinal submucosa patches in paediatric cardiovascular surgery. Eur J Cardiothorac Surg. (2013) 44:72–6. doi: 10.1093/ejcts/ezs638

PubMed Abstract | Crossref Full Text | Google Scholar

46. Zaidi AH, Nathan M, Emani S, Baird C, del Nido PJ, Gauvreau K, et al. Preliminary experience with porcine intestinal submucosa (CorMatrix) for valve reconstruction in congenital heart disease: histologic evaluation of explanted valves. J Thorac Cardiovasc Surg. (2014) 148:2216–25.e1. doi: 10.1016/j.jtcvs.2014.02.081

PubMed Abstract | Crossref Full Text | Google Scholar

47. Gerdisch MW, Shea RJ, Barron MD. Clinical experience with CorMatrix extracellular matrix in the surgical treatment of mitral valve disease. J Thorac Cardiovasc Surg. (2014) 148:1370–8. doi: 10.1016/j.jtcvs.2013.10.055

PubMed Abstract | Crossref Full Text | Google Scholar

48. Gerdisch MW, Boyd WD, Harlan JL, Richardson JB, Flack JE, Palafox BA, et al. Early experience treating tricuspid valve endocarditis with a novel extracellular matrix cylinder reconstruction. J Thorac Cardiovasc Surg. (2014) 148:3042–8. doi: 10.1016/j.jtcvs.2014.06.092

PubMed Abstract | Crossref Full Text | Google Scholar

49. Rosario-Quinones F, Magid MS, Yau J, Pawale A, Nguyen K. Tissue reaction to porcine intestinal submucosa (CorMatrix) implants in pediatric cardiac patients: a single-center experience. Ann Thorac Surg. (2015) 99:1373–7. doi: 10.1016/j.athoracsur.2014.11.064

PubMed Abstract | Crossref Full Text | Google Scholar

50. Hibino N, McConnell P, Shinoka T, Malik M, Galantowicz M. Preliminary experience in the use of an extracellular matrix (CorMatrix) as a tube graft: word of caution. Semin Thorac Cardiovasc Surg. (2015) 27:288–95. doi: 10.1053/j.semtcvs.2015.08.008

PubMed Abstract | Crossref Full Text | Google Scholar

51. Woo JS, Fishbein MC, Reemtsen B. Histologic examination of decellularized porcine intestinal submucosa extracellular matrix (CorMatrix) in pediatric congenital heart surgery. Cardiovasc Pathol. (2016) 25:12–7. doi: 10.1016/j.carpath.2015.08.007

PubMed Abstract | Crossref Full Text | Google Scholar

52. Nelson JS, Heider A, Si M-S, Ohye RG. Evaluation of explanted CorMatrix intracardiac patches in children with congenital heart disease. Ann Thorac Surg. (2016) 102:1329–35. doi: 10.1016/j.athoracsur.2016.03.086

PubMed Abstract | Crossref Full Text | Google Scholar

53. Hofmann M, Schmiady MO, Burkhardt BE, Dave HH, Hübler M, Kretschmar O, et al. Congenital aortic valve repair using CorMatrix® : a histologic evaluation. Xenotransplantation. (2017) 24:e12341. doi: 10.1111/xen.12341

Crossref Full Text | Google Scholar

54. Dobrilovic N, Soukas P, Sadiq I, Goldstein L, Raman J. Early complications of biologic extracellular matrix patch after use for femoral artery repair. J Vasc Surg. (2017) 65:705–10. doi: 10.1016/j.jvs.2016.07.131

PubMed Abstract | Crossref Full Text | Google Scholar

55. Ashfaq A, Brown T, Reemtsen B. Repair of complete atrioventricular septal defects with decellularized extracellular matrix: initial and midterm outcomes. World J Pediatr Congenit Heart Surg. (2017) 8:310–4. doi: 10.1177/2150135116684797

PubMed Abstract | Crossref Full Text | Google Scholar

56. Kelley TM, Kashem M, Wang H, McCarthy J, Carroll ND, Moser GW, et al. Anterior leaflet augmentation with CorMatrix porcine extracellular matrix in twenty-five patients: unexpected patch failures and histologic analysis. Ann Thorac Surg. (2017) 103:114–20. doi: 10.1016/j.athoracsur.2016.05.090

PubMed Abstract | Crossref Full Text | Google Scholar

57. Al Haddad E, LaPar DJ, Dayton J, Stephens EH, Bacha E. Complete atrioventricular canal repair with a decellularized porcine small intestinal submucosa patch. Congenit Heart Dis. (2018) 13:997–1004. doi: 10.1111/chd.12666

PubMed Abstract | Crossref Full Text | Google Scholar

58. Cox JL, Hammel JM, Radio SJ. Evaluation of cellular ingrowth within porcine extracellular matrix scaffolding in congenital heart disease surgery. Cardiovasc Pathol. (2019) 39:54–60. doi: 10.1016/j.carpath.2018.12.003

PubMed Abstract | Crossref Full Text | Google Scholar

59. Heinisch PP, Banz Y, Langhammer B, Stocker E, Erdoes G, Hutter D, et al. Histological analysis of failed submucosa patches in congenital cardiac surgery. Asian Cardiovasc Thorac Ann. (2019) 27:459–63. doi: 10.1177/0218492319858557

PubMed Abstract | Crossref Full Text | Google Scholar

60. Kiper C, Cua CL, Baker P, McConnell P. Mitral valve replacement in pediatrics using an extracellular matrix cylinder valve: a case series. Pediatr Cardiol. (2020) 41:1458–65. doi: 10.1007/s00246-020-02382-3

PubMed Abstract | Crossref Full Text | Google Scholar

61. Hu K, Siddiqi U, Lee B, Pena E, Schulz K, Vogel M, et al. Pediatric aortic valve repair: any development in the material for cusp extension valvuloplasty? J Card Surg. (2021) 36:4054–60. doi: 10.1111/jocs.15931

PubMed Abstract | Crossref Full Text | Google Scholar

62. Ebert N, McGinnis M, Johnson W, Kuhn EM, Mitchell ME, Tweddell JS, et al. Comparison of patch materials for pulmonary artery reconstruction. Semin Thorac Cardiovasc Surg. (2021) 33:459–65. doi: 10.1053/j.semtcvs.2020.09.011

PubMed Abstract | Crossref Full Text | Google Scholar

63. Sood V, Heider A, Rabah R, Si M-S, Ohye RG. Evaluation of explanted CorMatrix tyke extracardiac patches in infants with congenital heart disease. Ann Thorac Surg. (2021) 112:1518–22. doi: 10.1016/j.athoracsur.2020.06.151

PubMed Abstract | Crossref Full Text | Google Scholar

64. McCready RA, Kiell CS, Chugh AR, Rapp BM, Webb TH, Barksdale A, et al. Long-term results with CorMatrix extracellular matrix patches after carotid endarterectomy. J Surg Res. (2021) 262:21–6. doi: 10.1016/j.jss.2021.01.001

PubMed Abstract | Crossref Full Text | Google Scholar

65. Rescigno G, Hothi S, Bond C, Uddin M, Bhatti V, Billing JS. Cormatrix anterior leaflet augmentation of the tricuspid valve: midterm results. Heart Surg Forum. (2021) 24:E261–6. doi: 10.1532/hsf.3599

PubMed Abstract | Crossref Full Text | Google Scholar

66. Weis J, Geiger R, Kilo J, Zimpfer D. Cormatrix® for vessel reconstruction in paediatric cardiac surgery—a word of caution. Interact Cardiovasc Thorac Surg. (2022) 34:597–603. doi: 10.1093/icvts/ivab264

PubMed Abstract | Crossref Full Text | Google Scholar

67. Haney LC, Ahmed HF, Dani A, Chin P, Thangappan K, Madsen N, et al. More than 400 uses of an intestinal submucosal extracellular matrix patch in a congenital heart program. Ann Thorac Surg. (2022) 114:1475–83. doi: 10.1016/j.athoracsur.2021.06.087

PubMed Abstract | Crossref Full Text | Google Scholar

68. Padalino MA, Quarti A, Angeli E, Frigo AC, Vida VL, Pozzi M, et al. Early and mid-term clinical experience with extracellular matrix scaffold for congenital cardiac and vascular reconstructive surgery: a multicentric Italian study. Interact Cardiovasc Thorac Surg. (2015) 21:40–9. doi: 10.1093/icvts/ivv076

PubMed Abstract | Crossref Full Text | Google Scholar

69. Padalino MA, Castaldi B, Fedrigo M, Gallo M, Zucchetta F, Vida VL, et al. Porcine intestinal submucosa (CorMatrix) for semilunar valve repair in children: a word of caution after midterm results. Semin Thorac Cardiovasc Surg. (2016) 28:436–45. doi: 10.1053/j.semtcvs.2016.04.015

PubMed Abstract | Crossref Full Text | Google Scholar

70. DuBose JJ, Azizzadeh A. Utilization of a tubularized cormatrix extracellular matrix for repair of an arteriovenous fistula aneurysm. Ann Vasc Surg. (2015) 29:366.e1–4. doi: 10.1016/j.avsg.2014.10.018

PubMed Abstract | Crossref Full Text | Google Scholar

71. Yavuz K, Geyik S, Pavcnik D, Uchida BT, Corless CL, Hartley DE, et al. Comparison of the endothelialization of small intestinal submucosa, dacron, and expanded polytetrafluoroethylene suspended in the thoracoabdominal aorta in sheep. J Vasc Interv Radiol JVIR. (2006) 17:873–82. doi: 10.1097/01.RVI.0000217938.20787.BB

PubMed Abstract | Crossref Full Text | Google Scholar

72. Pavcnik D, Obermiller J, Uchida BT, Van Alstine W, Edwards JM, Landry GJ, et al. Angiographic evaluation of carotid artery grafting with prefabricated small-diameter, small-intestinal submucosa grafts in sheep. Cardiovasc Interv Radiol. (2009) 32:106–13. doi: 10.1007/s00270-008-9449-7

PubMed Abstract | Crossref Full Text | Google Scholar

73. Boni L, Chalajour F, Sasaki T, Snyder RL, Boyd WD, Riemer RK, et al. Reconstruction of pulmonary artery with porcine small intestinal submucosa in a lamb surgical model: viability and growth potential. J Thorac Cardiovasc Surg. (2012) 144:963–9.e1; discussion 969. doi: 10.1016/j.jtcvs.2012.07.024

PubMed Abstract | Crossref Full Text | Google Scholar

74. White JK, Agnihotri AK, Titus JS, Torchiana DF. A stentless trileaflet valve from a sheet of decellularized porcine small intestinal submucosa. Ann Thorac Surg. (2005) 80:704–7. doi: 10.1016/j.athoracsur.2004.08.063

PubMed Abstract | Crossref Full Text | Google Scholar

75. Padalino MA, Castellani C, Dedja A, Fedrigo M, Vida VL, Thiene G, et al. Extracellular matrix graft for vascular reconstructive surgery: evidence of autologous regeneration of the neoaorta in a murine model. Eur J Cardiothorac Surg. (2012) 42:e128–35. doi: 10.1093/ejcts/ezs462

PubMed Abstract | Crossref Full Text | Google Scholar

76. Soucy KG, Smith EF, Monreal G, Rokosh G, Keller BB, Yuan F, et al. Feasibility study of particulate extracellular matrix (P-ECM) and left ventricular assist device (HVAD) therapy in chronic ischemic heart failure bovine model. ASAIO J. (2015) 61:161–9. doi: 10.1097/MAT.0000000000000178

PubMed Abstract | Crossref Full Text | Google Scholar

77. Rosen M, Roselli EE, Faber C, Ratliff NB, Ponsky JL, Smedira NG. Small intestinal submucosa intracardiac patch: an experimental study. Surg Innov. (2005) 12:227–31. doi: 10.1177/155335060501200307

PubMed Abstract | Crossref Full Text | Google Scholar

78. Pavcnik D, Uchida BT, Timmermans HA, Corless CL, O'Hara M, Toyota N, et al. Percutaneous bioprosthetic venous valve: a long-term study in sheep. J Vasc Surg. (2002) 35:598–602. doi: 10.1067/mva.2002.118825

PubMed Abstract | Crossref Full Text | Google Scholar

79. Fallon A, Goodchild T, Wang R, Matheny RG. Remodeling of extracellular matrix patch used for carotid artery repair. J Surg Res. (2012) 175:e25–34. doi: 10.1016/j.jss.2011.11.001

PubMed Abstract | Crossref Full Text | Google Scholar

80. Pocaterra A, Romani P, Dupont S. YAP/TAZ functions and their regulation at a glance. J Cell Sci. (2020) 133:jcs230425. doi: 10.1242/jcs.230425

PubMed Abstract | Crossref Full Text | Google Scholar

81. Fraint HY, Richmond ME, Bacha EA, Turner ME. Comparison of extracellular matrix patch and standard patch material in the pulmonary arteries. Pediatr Cardiol. (2016) 37:1162–8. doi: 10.1007/s00246-016-1413-8

PubMed Abstract | Crossref Full Text | Google Scholar

Keywords: porcine small intestinal submucosal extracellular matrix, small intestinal submucosal extracellular matrix, CorMatrix, cardiovascular surgery, tissue engineering, bio scaffold

Citation: Bruun VJ, Jensen LL, Hasenkam JM and Jedrzejczyk JH (2025) Tissue response and clinical outcomes after cardiovascular use of porcine small intestinal small intestinal submucosal extracellular matrix: a systematic review. Front. Cardiovasc. Med. 12:1532157. doi: 10.3389/fcvm.2025.1532157

Received: 14 February 2025; Accepted: 12 June 2025;
Published: 25 June 2025.

Edited by:

Tamer M. A. Mohamed, Baylor College of Medicine, United States

Reviewed by:

Dominga Iacobazzi, University of Bristol, United Kingdom
Ngan F. Huang, Stanford University, United States
Marianna Scuglia, KU Leuven, Belgium

Copyright: © 2025 Bruun, Jensen, Hasenkam and Jedrzejczyk. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Johannes H. Jedrzejczyk, am9oamVkQGNsaW4uYXUuZGs=

ORCID:
Villads Juul Bruun
orcid.org/0009-0000-2957-4735
Line Lyngbak Jensen
orcid.org/0009-0000-2607-3142
J. Michael Hasenkam
orcid.org/0000-0001-7960-1172
Johannes H. Jedrzejczyk
orcid.org/0000-0001-5029-9117

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