ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Genetics, Epigenetics and Chromosome Biology
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1555316
This article is part of the Research TopicEpigenetics, Transcriptomics and Epitranscriptomic – Deal With Old Friends or New Direction of Regulation?View all 4 articles
Fine mapping and characterization of Fusarium wilt (Fusarium oxysporum f. sp. benincasae) resistance gene Fob1(t) in wax gourd (Benincasa hispida Cogn.)
Provisionally accepted
Fahuo Li1,2
Jian Qin1,2Jingying Li1,2Liang Cheng1,2Xuehan He3Xiaohui Zhong1,2Yangpeng Mo1,2Han Liang1,2
Peng Wang3Yan Li1,2Yongguan Wu1,2*- 1Guangxi Key Laboratory of Vegetable Breeding and New Technology Development, Nanning, China
- 2Vegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, China
- 3College of Agriculture, Guangxi University, Nanning, Guangxi Zhuang Region, China
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Fusarium wilt (FW), caused by the plant fungus Fusarium oxysporum, yielding severe economic losses in wax gourd (Benincasa hispida Cogn.). Developing disease-resistant varieties is an effective measure. Currently, no reports exist on gene localization or cloning of FW resistance in wax gourd. However, our team has identified a FW resistance gene and plans to apply it in resistant varieties. In this study, we used bulked segregant analysis sequencing and quantitative trait loci detection on 1304 inbred lines derived from resistant GD68 and susceptible HM25 parents to identify FW resistance genes. A resistance locus was successfully identified between 3M13.385 and 3M16.869 markers on chromosome 3, named Fob1(t). Fine mapping between markers 3M15.904 and 3M16.373 (469 kb apart) identified 22 candidate genes. The transcriptome, sequencing comparison and qRT-PCR analysis suggested that the endochitinase gene Bch03G006380 is the resistance gene, with significant expression differences between the parents and a one-base mutation in the first exon. The study also revealed the roles of Fob1(t) in plant hormones, transcription factors, phenylpropane metabolism, oxidation-reduction, disease progression, enzymes, and cell wall modification pathway, enhancing FW resistance in GD68. Finally, we identified two closely linked insertion-deletion markers that can assist in transfer and utilization of the Fob1(t) gene, significantly improving the screening rate of positive individual plants and reducing breeding time.
Keywords: Wax gourd, Fusarium Wilt, bulked segregant analysis sequencing (BSA-seq), gene mapping, marker-assisted selection (MAS)
Received: 04 Jan 2025; Accepted: 15 Apr 2025.
Copyright: © 2025 Li, Qin, Li, Cheng, He, Zhong, Mo, Liang, Wang, Li and Wu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Yongguan Wu, Guangxi Key Laboratory of Vegetable Breeding and New Technology Development, Nanning, China
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