ORIGINAL RESEARCH article

Front. Plant Sci.

Sec. Plant Metabolism and Chemodiversity

Volume 16 - 2025 | doi: 10.3389/fpls.2025.1555374

Transcriptomic Reveals Key Genes and Regulatory Pathways in Galactomannan Biosynthesis in Gleditsia sinensis Polysaccharide Gum

Provisionally accepted
  • 1Guizhou University of Traditional Chinese Medicine, Guiyang, China
  • 2Guizhou Minzu University, Guizhou University of Traditional Chinese Medicine, Guiyang, China

The final, formatted version of the article will be published soon.

The Gleditsia sinensis Lam. polysaccharide gum contained in G. sinensis seeds is not only an additive specified in national food standards, but also an important strategic resource for industrial raw materials such as oil and natural gas in China.The main component of G. sinensis polysaccharide gum is galactomannan (GM). To date, most studies have focused on the structural modification and component separation of the polysaccharide gum of G. sinensis seeds, with only a few reports on the regulatory genes involved in its formation.Methods: Transcriptome analysis was performed to assess the gene networks associated with GM synthesis in G. sinensis seeds at four stages: 6, 9, 12, and 16 weeks after flowering.The result indicated that differential expression analysis identified 20 unigenes linked to five critical enzymes in the GM biosynthesis pathway. Further pathway enrichment analysis demonstrated that fructokinase, galactose gyrase, inositol galactoside synthase, phosphogalactosyltransferase, and raffinose synthase play pivotal roles in GM biosynthesis, positively regulating its production.The results of this study provide new ideas for the research of GM biosynthesis related genes in G. sinensis and enhance the potential application prospects in genetic engineering.

Keywords: Gleditsia sinensis Lam., Galactomannan, Transcriptome, biosynthesis, unigenes enrichment analysis

Received: 04 Jan 2025; Accepted: 07 Jul 2025.

Copyright: © 2025 Zhang, Yang, Wang, Xu, Du and Wu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Linlin Du, Guizhou University of Traditional Chinese Medicine, Guiyang, China
Hongmei Wu, Guizhou University of Traditional Chinese Medicine, Guiyang, China

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