ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Systems and Synthetic Biology
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1566354
Integrated miRNAome, transcriptome, and degradome analyses reveal the role of miRNA-mRNA Modules in oridonin biosynthesis in Isodon rubescens
Provisionally accepted
- 1Henan University of Chinese Medicine, Zhengzhou, China
- 2Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao, Zhengzhou, China
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Isodon rubescens contains many bioactive diterpenoids, especially oridonin, which are used both as medicines and drinks. However, the structure and content of the diterpenoids in I. rubescens vary greatly in response to different ecological environments. MicroRNAs (miRNAs) play a pivotal role in the biosynthesis of secondary metabolites; but their roles in I. rubescens are obscure. In this study, a total of 1634 miRNAs were identified from 9 miRNAome libraries of three I. rubescens ecotypes, which contained various contents of oridonin, lasiodonin, and rosthorin A.Furthermore, 99 differentially expressed miRNAs (DEMs) and 8180 differentially expressed mRNAs (DEGs) were obtained across the three I. rubescens ecotypes, and the expressions of selected DEMs and DEGs were verified via RT-qPCR. A total of 8928 miRNA-mRNAs networks were identified by degradome analysis, and 23 miRNA-mRNA modules were enriched in the terpenoid biosynthesis pathway.Additionally, 92 negatively correlated DEM-DEG modules were identified through integrated miRNAome, transcriptome, and degradome analyses, ath-miR858b_1ss21GA-MYB and ath-miR408-3p_L-1R+1-CYP72A219 modules were likely involved in oridonin biosynthesis in I. rubescens. Furthermore, the negative regulation of ath-miR858b_1ss21GA targeted MYB was validated through a dualluciferase reporter system. This study revealed that the miR858b_1ss21GA-MYB module most likely participates in oridonin biosynthesis in I. rubescens.
Keywords: Isodon rubescens, miR858b_1ss21GA-MYB module, oridonin biosynthesis, Multiomics analysis, Dual-luciferase reporter assay
Received: 11 Feb 2025; Accepted: 21 May 2025.
Copyright: © 2025 Zhu, Zhang, Guo, Xu and Dai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Lili Zhu, Henan University of Chinese Medicine, Zhengzhou, China
Liping Dai, Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao, Zhengzhou, China
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