ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Pathogen Interactions
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1571893
Transcriptome and Metabolome Profiling Reveal the Chlorogenic Acid as a Resistance Substance for Rice against the White-backed Planthopper Sogatella furcifera (Horváth)
Provisionally accepted
Wenqi Xie1
Wencai Tao1
Zhang Huilai1Juntao Luo2Xiaoyue Deng1,3Deqiang Li4
Qing Li1Haijian Wang1Yanli Yue1Surong Jiang1Chunxian Jiang1*- 1College of Agronomy, Sichuan Agricultural University, Chengdu, China
- 2Sorghum Research Institute of the Sichuan Academy of Agricultural Science, Deyang, China
- 3Emeishan Agricultural and Rural Bureau, Emeishan, China
- 4State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan Province, China
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The white-backed planthopper (WBPH), Sogatella furcifera (Horváth) is a major migratory pest of rice, making research on rice resistance to WBPH essential for rice breeding and pest management. This study compared the resistance of susceptible rice TN1 and resistant rice KL35 to WBPH by analyzing antixenosis, antibiosis, and tolerance. We also conducted transcriptome and metabolome analysis to identify the defensive compounds against the WBPH and regulatory genes in KL35. The results indicated that KL35 exhibited significant antixenosis and tolerance to WBPH, markedly prolonging developmental duration and reducing fecundity. Metabolomic analysis identified 15 core metabolites, among which chlorogenic acid (CGA) content in KL35 was significantly higher than in TN1 both before and after WBPH feeding. Integrated transcriptomic and metabolomic analyses showed that the flavonoid biosynthetic pathway was a key anti-pest pathway in KL35. Additionally, 3 genes involved in the CGA synthesis were significantly upregulated: cinnamate 4-hydroxylase gene (Os05g0320700), 4-coumarate CoA ligase (Os02g0697400), and hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase gene (novel.1556). Exogenous application of CGA to TN1 enhanced its tolerance and antixenosis to WBPH, significantly decreasing WBPH’s survival and mean dry weight. These findings suggest that CGA is an important resistance substance against WBPH, offering a new perspective for breeding resistant rice.
Keywords: White-Backed Planthopper, rice, Transcriptome, Metabolome, Chlorogenic Acid, Resistance
Received: 07 Feb 2025; Accepted: 21 Apr 2025.
Copyright: © 2025 Xie, Tao, Huilai, Luo, Deng, Li, Li, Wang, Yue, Jiang and Jiang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Chunxian Jiang, College of Agronomy, Sichuan Agricultural University, Chengdu, China
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