ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Metabolism and Chemodiversity
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1577695
This article is part of the Research TopicMolecular Architects of the Green World: Genetic, Epigenetic, and Transcriptional Regulation of Plant Metabolism and Chemo-diversityView all articles
Integrated physiological, microbial, and metabolomics analyses revealed the differences in different varieties of Paeonia lactiflora Pall
Provisionally accepted
Shuang Wu1
Beijing Tian1Chenggang Shan1Xin Wang1xinjing Xie2hongqing xie1xiuwen jia1Feng Zhang1Jinlong Han1*- 1Shandong Academy of Agricultural Sciences, Jinan, China
- 2Shandong Xieshi Chinese Herbal Pieces Co., Ltd.,, Heze, China
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Paeonia lactiflora Pall (P. lactiflora) is a perennial herb with high medicinal and economic value. In the growth process of P. lactiflora, the plant's root secondary metabolism is intricately linked to the microbial communities that surround it. However, few systematic studies have reported the changes in the microbiome and metabolites during P. lactiflora cultivation thus far. In this study, amplicon sequencing technology was used to determine the difference in rhizosphere microorganisms of P. lactiflora. The non-targeted metabolomics method was used to determine the changes in root metabolites, and the relationship between microorganisms and metabolites was demonstrated by coexpression network analysis. The paeoniflorin content (PC) was determined by HPLC. The total phenol content (TPC) was determined by the Folin-Ciocalteu method, and the total flavonoid content (TFC) was determined by the NaNO2-Al (NO3)3 method. The antioxidants were evaluated with the DPPH, ABTS, and FRAP methods. Results showed that Proteobacteria had the highest relative abundance among all phyla, Halomonas had the highest relative abundance among all genera. The results of metabolomics showed that 693 metabolites and 207 differential metabolites were detected in the four groups, which were mainly enriched in the biosynthesis of phenylpropanoids, phenylpropanoid biosynthesis, taste transduction, central carbon metabolism in cancer, and biosynthesis of plant secondary metabolites. The results also showed that the PC, TPC, TFC, and antioxidant capacity of the white P. lactiflora group were higher than those of the other groups. This study revealed the differences between different varieties of P. lactiflora and provided theoretical support for breeding and data reference for improving the quality of P. lactiflora by regulating microbial species.
Keywords: Paeonia lactiflora Pall., Metabolome, microbiome, active ingredient, antioxidant activity
Received: 16 Feb 2025; Accepted: 05 May 2025.
Copyright: © 2025 Wu, Tian, Shan, Wang, Xie, xie, jia, Zhang and Han. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Jinlong Han, Shandong Academy of Agricultural Sciences, Jinan, China
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