Exploring the structural variability within developing wheat grains using autofluorescence multispectral imaging at the macroscopic scale

Anne-Laure Chateigner-Boutin^*Fabienne GuillonMarie-Françoise Devaux

• Institut National de recherche pour l’agriculture, l’alimentation et l’environnement (INRAE), Paris, France

The wheat grain undergoes major changes in dimensions, shape and composition during its development, and the outer tissues of the grain contribute to these modifications. In order to explore the spatial and temporal variability within the developing wheat grain, a series of 40 autofluorescence multispectral images was acquired for whole grain sections at 4 development stages. The images were analyzed using principal component analysis of large image series (large PCA). This chemometric method enables the quantification of the variability, its localization, highlighting the associated spectral characteristics and their attribution to chemical compounds. Regional and temporal heterogeneity was revealed. Among the new information, the lignification of cell walls in pericarp tissues first appeared patchy and become later homogeneous. Polar differences have been found in aleurone cell walls for phenolic compounds. This work demonstrates that multispectral autofluorescence analysis of plant organ sections combined with large PCA is a powerful tool for studying the relationship between tissue chemical composition, its variability and the internal structure of organs at the macroscopic scale.