ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Development and EvoDevo
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1581069
This article is part of the Research TopicApical Meristem Manipulation for Biotechnology Approaches in PlantsView all 3 articles
Tissue culture requirements of the evergreen broad-leaved plant Illicium henryi D
Provisionally accepted
- 1Northwest A&F University, Xianyang, China
- 2Northeast Forestry University, Harbin, Heilongjiang Province, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Illicium henryi Diels, a rare wild plant species with limited natural distribution and challenging cultivation requirements, has received little attention regarding its introduction and propagation techniques. Given its restricted range, ecological vulnerability, and potential medicinal and ornamental value, the development of efficient propagation methods is essential for its conservation and sustainable utilization. This study aims to establish an efficient protocol for the aseptic plantlet production of I. henryi using tissue culture technology. Semi-lignified stem segments with buds, young leaves, sprouting buds, and young shoots bearing buds from the current year were used as explants. The effects of different sterilization methods and explant types on disinfection efficiency were systematically investigated. Based on these results, suitable basal media were selected for bud induction and leaf-derived callus induction. Furthermore, the roles of plant growth regulators, such as 6-BA, NAA, and IBA, were evaluated in the stages of bud induction, callus formation, and proliferation. This study successfully achieved the in vitro propagation of plantlets of I. henryi, offering a practical method for its artificial propagation in introduced habitats. Additionally, it established a stable and efficient regeneration system for both bud-bearing stem segments and leaf-derived callus, providing a solid foundation for future research and tissue culture applications in I. henryi and related species within the Illicium genus.MS Murashige and Skoog, 0.5x MS 0.5x Murashige & Skoog medium, WPM Lignified plant medium, 6-BA 6-Benzylaminopurine, NAA 1-naphthaleneacetic acid, IBA Indole-3-butyric acid, KT 6-Furfurylamino-purine, AC Activated carbon.Acknowledgements The authors would like to thank the review team for their support in this study.
Keywords: Callus1, Evergreen broad-leaved plant2, Illicium henryi3, In vitro tissue culture4, Plant growth regulator5 PPL: Conceptualization, methodology, investigation, Writing -Original Draft. YY: Data Curation
Received: 24 Feb 2025; Accepted: 18 Aug 2025.
Copyright: © 2025 Li, Yang, Jiang, Chen, Xue, Ji and Ji. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Wenli Ji, Northwest A&F University, Xianyang, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.