ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Nutrition
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1586547
This article is part of the Research TopicPlant Responses to Phosphorus and Nitrogen Starvation: Genetic Insights and Agricultural InnovationsView all 6 articles
Genome-wide identification of GDPD gene family in foxtail millet (Setaria italica L.) and functional characterization of SiGDPD14 under low phosphorus stress
Provisionally accepted
- Henan University of Science and Technology, Luoyang, China
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Glycerophosphodiester phosphodiesterase (GDPD) catalyzes the hydrolysis of glycerophosphodiesters into sn-glycerol-3-phosphate (G-3-P) and corresponding alcohols, which is integral to various physiological processes in plants. However, our comprehension of the GDPD gene family in foxtail millet (Setaria italica L.) remains limited and unclear. This study aimed to identify and predict the function of GDPD gene family members in foxtail millet through a comprehensive genome-wide analysis.14 SiGDPD genes were identified in the foxtail millet genome. Phylogenetic analysis categorized SiGDPD proteins into five groups. Promoter regions of SiGDPD genes contained multiple cis-acting elements related to light response, hormone regulation, and stress response. Phylogenetic and collinearity analyses demonstrated conservation of GDPD proteins among foxtail millet, sorghum, rice, and maize, with the SiGDPD gene family undergoing purifying selection during evolution.Tissue differential expression analysis revealed distinct expression patterns of SiGDPD genes across various tissues, showing spatiotemporal expression characteristics.Under low phosphorus stress, the expression levels of SiGDPD3 and SiGDPD14 significantly increased, while SiGDPD1, SiGDPD5, SiGDPD6, and SiGDPD11 showed significant decreases (p<0.05).To identify the function of SiGDPD14, an over-expressed transgenic Arabidopsis was constructed. After treatment with low phosphorus stress, the germination rate, root length, and root surface area were measured.The results showed that transgenic Arabidopsis thaliana plants overexpressing SiGDPD14 exhibited enhanced tolerance to low phosphorus stress.Taken together, the results of this study provide valuable information for further studies on candidate SiGDPD genes involved in the phosphate deficiency response in foxtail millet.
Keywords: foxtail millet, GDPD, gene family, Low phosphorus stress, over-expression, functional characterization
Received: 03 Mar 2025; Accepted: 26 May 2025.
Copyright: © 2025 Meng, Guo, Wang, Yang and Du. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Chaomin Meng, Henan University of Science and Technology, Luoyang, China
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