ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Breeding
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1588460
Identification of three novel QTL for resistance to highly aggressive Canadian strains of Plasmodiophora brassicae in rutabaga cultivar ECD10
Provisionally accepted
- Saskatoon Research and Development Centre, Agriculture and Agri-Food Canada (AAFC), Saskatoon, Saskatchewan, Canada
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Plasmodiophora brassicae, the causal pathogen of clubroot, has emerged as a significant threat to the production of Canada's most important oilseed crop canola. The B. napus rutabaga cultivar 'Wilhelmsburger' (ECD10) has shown a high resistance level to Canadian P. brassicae strains. This study utilized genotyping-by-sequencing (GBS) to identify single-nucleotide polymorphism (SNP) markers for mapping QTL related to resistance to 11 P. brassicae strains in a BC1/BC1S1 population. The short reads from the GBS were assembled to the Chinese canola cultivar 'ZS11' reference genome. A genetic map spanning 1,812.6 cM was developed using 1,049 non-redundant SNPs identified from 92 BC1 plants. Three quantitative trait loci (QTL) were identified: Rcr13 on chromosome A02, Rcr11 on A08, and Rcr_C03-4 ECD10 on C03. Rcr13 was related to resistance to four strains (2B, 8J, 5L, and 5X), explaining 14.2% to 81.7% of the phenotypic variation explained (PVE). Rcr11 was associated with resistance to eight strains (3A, 2B, 5C, 3D, 8E, 5G, 3O, and 8P), explaining 20.2% to 83.2% of the PVE.Rcr_C03-4 ECD10 was linked to resistance against strain 5C, explaining 21.5% of the PVE.Unclassified/Non classifié Thirteen genes that encode proteins related to disease resistance were found as candidate genes for three QTL. The syntenic regions of the QTL were also examined with the reference genomes of the B. rapa 'Chiifu' and the B. oleracea 'D134'. Differential reactions of the B. napus line carrying Rcr11 or Rcr13 with those carrying the previously identified CR genes Rcr3 and Rcr9 or Rcr8 were found with the purified P. brassicae isolates. This is the first report of rutabaga-derived QTL effective against Canadian pathotypes that overcome 'Mendel' resistance.
Keywords: Brassica napus, Plasmodiophora brassicae, Clubroot resistance, genotyping-by-sequencing (GBS), GBS-SNP-CROP, genetic mapping, QTL, candidate gene
Received: 05 Mar 2025; Accepted: 15 May 2025.
Copyright: © 2025 Karim and Yu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Fengqun Yu, Saskatoon Research and Development Centre, Agriculture and Agri-Food Canada (AAFC), Saskatoon, S7N 0X2, Saskatchewan, Canada
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.