ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Bioinformatics
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1589847
This article is part of the Research TopicEvolutionary Dynamics, Functional Variation and Application of Plant Organellar GenomeView all 20 articles
The complete mitochondrial genome of Cenchrus fungigraminus indicates structural dynamics and sequence divergences in Poaceae family
Provisionally accepted
- Nanchang Normal University, Nanchang, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Background: Cenchrus fungigraminus is a perennial grass species, which is used in the cultivation of edible fungi, helping to resolve land use conflicts between edible fungus cultivation and forest production. It can also be utilized forbioethanol production and fiberboard manufacturing. Currently, no report was released on the mitochondrial genome. Thus, the C. fungigraminus mitochondrial genome was assembled and annotated utilizing a combination of Illumina and PacBio sequencing data.The assembled genome measures 404,398 bp and exhibits a linear structure. The GC content is 43. 98%, and 64 characteristic genes were identified, containing 34 protein-coding, six rRNA, 22 tRNA and two pseudo-genes, with 17 genes containing 30 introns. The mitochondrial genome codon usage displayed a significant A/T preference. A total of 235 dispersed, 93 simple sequence, and 38 tandem repeats were detected. Furthermore, 34 sequence fragments (17,634 bp) were transferred from the chloroplast to the mitochondria, accounting for 4.36% of the complete mitochondrial genome. The gene rps2 was identified as having the highest polymorphism. Additionally, these genes (ccmB, nad2, and rps7) were influenced by positive selection. Among the reported Poaceae mitochondrial genomes, the genome sizes range from 381,065 to 704,100 bp, with GC content ranging from 42.27% to 44.43%. Three genes (sdh3, rps10, and rpl6) are missing in the Poaceae mitochondrial genome, indicating significant rearrangements in the overall genome structure and low homology. It was shown that C. fungigraminus is most closely related to Saccharum officinarum within the Poaceae family by Phylogenetic analysis. The interspecific Ka/Ks comparison indicated that 28 protein-coding genes have a Ka/Ks ratio < 1.Therefore, the mitochondrial genome data of C. fungigraminus was enriched and a theoretical basis was provided for germplasm evaluation and new variety utilization.
Keywords: C. fungigraminus, RPS2, Ka/Ks, phylogenetic analysis, mitochondria 1. Introduction
Received: 08 Mar 2025; Accepted: 15 May 2025.
Copyright: © 2025 Sheng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Wentao Sheng, Nanchang Normal University, Nanchang, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.