ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Symbiotic Interactions
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1595870
This article is part of the Research TopicGenetics and Genomics of Emerging and Multifactorial Stresses Affecting Plant Survival and Associated Plant MicrobiomesView all 16 articles
Comprehensive metabolomic and microbial analysis of tobacco rhizosphere soil responses to crop rotation and fertilization
Provisionally accepted
Xinyu Liu1Xuanxuan Chen2Xianzhi Wu3Ying Qiao1Fan Yang1Hao Li2Chuanzong Li2Yong Yang1Chunlei Yang2*
Jun Yu2
Pan Luo1*- 1Hubei University, Wuhan, China
- 2Tobacco Research Institute of Hubei Province, Wuhan, Hebei Province, China
- 3Bijie Academy of Agricultural Sciences, Bijie, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Tobacco (Nicotiana tabacum L.) is a crucial Solanaceae crop globally, but its continuous cultivation can lead to soil degradation. Crop rotation offers numerous benefits, including enhanced soil fertility, improved microbial communities, and pest control. However, how different tobacco planting systems specifically reshape rhizosphere metabolite profiles and regulate microbial diversity remains unclear. Here, we analyzed soil samples from four tobacco cropping systems using non-targeted metabolomics, 16S rRNA and ITS sequencing. The results revealed distinct changes in soil metabolite profiles and microbial communities under different treatments. We identified significant alterations in lipid metabolism, amino acid biosynthesis, and secondary metabolite pathways, which influence soil microbial populations and tobacco plant health. Lipid metabolites, including fatty acids and eicosanoids, were particularly notable for their roles in microbial signaling and plant defense. Furthermore, microbial gene abundance analysis indicated that different treatments fostered unique microbial populations, including increased arbuscular mycorrhizal fungi and saprotrophic fungi, which support nutrient cycling and plant growth. These findings highlight the critical interplay between soil metabolites, microbial diversity, and plant productivity, offering insights into optimizing tobacco cropping systems for improved soil health and sustainable agricultural practices.
Keywords: Tobacco, Crop rotation, rhizosphere metabolites, Microbial Diversity, soil fertility
Received: 18 Mar 2025; Accepted: 06 May 2025.
Copyright: © 2025 Liu, Chen, Wu, Qiao, Yang, Li, Li, Yang, Yang, Yu and Luo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Chunlei Yang, Tobacco Research Institute of Hubei Province, Wuhan, Hebei Province, China
Pan Luo, Hubei University, Wuhan, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.