ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Crop and Product Physiology
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1598930
Identification of a Locus Associated with Chlorosis and Antioxidant Capacity using RNA-seq and BSA-seq in Soybean [Glycine max (L.) Merr]
Provisionally accepted
Duo Lv1,2,3
Lihua Zhu1,2,3Jiaqi You1,2,3
Biting Cao1,2,3Hongjuan Yang1,2,3Weihong Gu1,2,3
Qingzhu LI1,3,4Chaohan Li1,2,3*Yuan Yuan1,2,3- 1Shanghai Academy of Agricultural Sciences, Shanghai, China
- 2Horticulture Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
- 3Shanghai Key Laboratory of Horticultural Technology, Shanghai Academy of Agricultural Sciences, Shanghai, China
- 4Forestry and Fruit Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, Shanghai Municipality, China
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Plant coloration is an important trait in soybean (Glycine max), and its underlying molecular mechanisms are complex. In this study, we identified a chlorotic mutant, el-5y, in the wild-type soybean cultivar VS-5. Compared with those in VS-5, the capacities for photosynthesis, photosynthetic pigment synthesis, and antioxidant production in the el-5y mutant were significantly decreased, leading to a significant decrease in production. Transmission electron microscopy showed that the el-5y mutant cells were malformed, and abnormal lamellae and starch granules in the chloroplasts were significantly increased. Transcriptome analysis indicated that the DEGs between VS-5 and the el-5y mutants were mainly enriched in pathways related to the reduction-oxidation system, iron ion homeostasis, and auxin signaling transport.Based on the bulked segregant analysis data, the el-5y mutant was mapped to a 1.16-Mb interval on chromosome 11. The results of this study serve as a basis for further explorations into the molecular mechanisms that regulate plant coloration.
Keywords: Glycine max, Etiolation, Photosynthesis, Bulked segregant analysis, 1-aminocyclopropane-1-carboxylate synthase
Received: 24 Mar 2025; Accepted: 05 Jun 2025.
Copyright: © 2025 Lv, Zhu, You, Cao, Yang, Gu, LI, Li and Yuan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Chaohan Li, Shanghai Academy of Agricultural Sciences, Shanghai, China
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