ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Systematics and Evolution
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1599596
This article is part of the Research TopicEvolutionary Dynamics, Functional Variation and Application of Plant Organellar GenomeView all 24 articles
Characterization and phylogenetic analysis of the complete mitochondrial genome sequence of Lagenaria siceraria, a cucurbit crop
Provisionally accepted
- 1Horticulture Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
- 2Zhuanghang Comprehensive experiment station, Shanghai Academy of Agricultural Sciences, Shanghai, China
- 3Fengxian District Agricultural Technology Extension Center, Shanghai, China
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Bottle gourd (Lagenaria siceraria)belongs to cucurbit crop and hasunique semi-autonomous organelle genome. Using Illumina short-read and Nanopore long-read sequencing data, we sequenced and annotated the complete mitochondrial genome of L. siceraria. And a comparative phylogenetic analysis was conducted with its close relatives. The mitochondrial genome of bottle gourd is a circular sequence of 357,496 bp with a GC content of 45.03%. It contains 63 genes, including 34 mRNAs, 24 tRNAs, 4 rRNAs, and 1 pseudogene. The rps19 gene is present, but rpl10 is absent.22,294 bp (6.24%) are repetitive sequences. 497 RNA editing sites were identified. 45 homologous fragments (40,579 bp, 11.35%) were shared with the chloroplast genome.. Phylogenetic analysis revealed that C. maxima, C. sativus, C. lanatus, and L. acutangula are closely related to bottle gourd. Gene arrangement analysis indicated that L. acutangula exhibits the highest collinearity with L. siceraria compared to other cucurbit crops. However, genome size and repetitive sequences are most similar to watermelon.. Nearly all Ka/Ks ratios <1.0 suggest stabilizing selection in protein-coding genes. These findings provide a foundation for further understanding the evolutionary relationships within cucurbit crops.
Keywords: Lagenaria siceraria, Mitochondrial Genome, phylogenetic analysis, cucurbit, Evolutionary analysis
Received: 27 Mar 2025; Accepted: 27 Jun 2025.
Copyright: © 2025 Du, Wang, Tang, Wu, Yang, Zhang, Zhang and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Zhaohui Zhang, Zhuanghang Comprehensive experiment station, Shanghai Academy of Agricultural Sciences, Shanghai, China
Na Liu, Horticulture Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
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