ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Nutrition
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1600887
This article is part of the Research TopicHarnessing Plant–Microbe Interactions to Improve Nitrogen Use Efficiency and Plant-Soil Health for Sustainable AgricultureView all 12 articles
Different Forms of Nitrogen Uptake in Tobacco Promoted by the Arbuscular Mycorrhizal Fungi
Provisionally accepted
- 1Anshun University, Anshun, China
- 2Guizhou University, Guiyang, Guizhou Province, China
- 3Guizhou Tobacco Company, Guiyang, Guizhou Province, China
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Persistent limitations in nitrogen (N) assimilation efficiency have emerged as a critical constraint in advancing the phytochemical quality of cultivated tobacco (Nicotiana tabacum L.). Arbuscular mycorrhizal fungi (AMF), forming obligate symbiotic associations with over 80% of terrestrial vascular plant species, significantly enhance host plant performance through improved rhizospheric nutrient mobilization. This mutualistic relationship facilitates enhanced acquisition of both macronutrients (particularly phosphorus and N) and water, thereby substantially decreasing agricultural dependence on synthetic fertiliser inputs. Building upon these premises, the present study was carried out to investigate the effects of different forms of nitrogen on the infestation rate and biomass of tobacco plants after inoculation with AMF, as well as the differences in the uptake of different forms of nitrogen by tobacco plants mediated by AMF, using the isotope 15 N labelling method. The study revealed significant variations in the uptake of various nitrogen forms by AMF. Under mixed nitrogen source conditions, (NH4)2SO4, KNO3, and glutamine (Glu) constituted 48.61%, 36.10%, and 15.29% of total nitrogen uptake, respectively. Notably, AMF exhibited a preferential uptake hierarchy for NH4 + , demonstrating 1.35-fold and 2.94-fold higher absorption rates compared to NO3 -and Glu. Furthermore, 15 N isotopic tracing analysis confirmed active Glu assimilation by AMF, as evidenced by significantly elevated 15 N-Glu uptake in labeled treatments relative to non-labeled controls. These findings collectively suggest that AMF symbiosis modifies tobacco plants' nutritional preferences among distinct nitrogen forms. This study establishes a theoretical foundation for optimizing nitrogen utilization efficiency and enhancing agronomic productivity in tobacco cultivation systems.
Keywords: arbuscular mycorrhizal fungi, Nitrogen, Tobacco, absorbance mismatch, 15N
Received: 27 Mar 2025; Accepted: 05 Jun 2025.
Copyright: © 2025 Xie, Song, Lu, Hu, Pan, Xu and Xue. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Yanlan Xie, Anshun University, Anshun, China
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