METHODS article
Front. Plant Sci.
Sec. Plant Development and EvoDevo
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1601397
This article is part of the Research TopicAdvanced Imaging in Plants: Exploring Development and FunctionView all 6 articles
A long term time lapse microscopy technique for Arabidopsis roots
Provisionally accepted
- New York University, New York City, United States
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Time lapse microscopy is a transformative technique for plant cell and developmental biology. Light sheet microscopy, which manipulates the amount of light a sample is exposed to in order to minimize phototoxicity and maximize signal intensity, is an increasingly popular tool for time lapse imaging. However, many light sheet imaging systems are not designed with the unique properties of plant samples in mind. Recent advances have decreased the cost and increased the technical accessibility of light sheet microscopy, but plant samples still require special preparation to be compatible with these new systems. Here, we apply a novel light sheet microscopy system to regenerating plantArabidopsis roots. damaged via laser ablation. To adapt this system for plantArabidopsis roots we establish a new protocol for sample mounting, as well as an automated root tip tracking system that requires no additional proprietary software. The methods presented here can be used to increase researcher access to long-term timelapse imaging in plantArabidopsis biology.
Keywords: Microscopy, Time lapse microscopy, root development, Light sheet microscope, Regeneration
Received: 27 Mar 2025; Accepted: 23 May 2025.
Copyright: © 2025 Lee, Rahni and Birnbaum. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Kenneth D Birnbaum, New York University, New York City, United States
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