ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Physiology
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1603423
This article is part of the Research TopicBiochemical and Physiological Insights into Plant Adaptation and Resilience Under Abiotic StressesView all articles
PpSKα boosts chilling tolerance by activating PpTrxh9 in peach fruit
Provisionally accepted
Caifeng Jiao1*
Jing Sun2- 1Anhui Agricultural University, Hefei, China
- 2Nanjing University of Finance and Economics, Nanjing, Jiangsu Province, China
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Cold chain system is extensively used to lower the quality deterioration in postharvest fruits.However, the occurrence of chilling injury (CI) is a common phenomenon in peach fruit, consequently compromising their market value. The cold tolerance was boosted by glycine betaine (GB) supplementation. GB treatment promoted the thioredoxin h9 (PpTrxh9) expression and ameliorated the oxidative injury. SHAGGY-related protein kinase α (PpSKα) was verified to be a protein interacting with PpTrxh9 by using assays of yeast two-hybrid, coimmunoprecipitation, pull-down and bimolecular fluorescence complementation. GB treatment elevated the PpSKα expression. Overexpression of PpSKα in tomato fruit reduced the cold sensitivity and oxidative damage, whereas virus-induced gene silencing of PpSKα in peach fruit aggravated the CI progression and oxidative damage. PpSKα was found to phosphorylate PpTrxh9 in kinase assay. Moreover, the PpSKα-overexpressed tomato fruit exhibited the higher SlTrxh9 expression, whereas the PpSKα-RNAi peach fruit exhibited the lower PpTrxh9 expression. Taken together, PpSKα lowered the development of CI by improving PpTrxh9 expression in peach fruit.
Keywords: Chilling tolerance, SHAGGY-related protein kinase α, thioredoxin h9, Oxidative damage, Peach fruit
Received: 31 Mar 2025; Accepted: 12 May 2025.
Copyright: © 2025 Jiao and Sun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Caifeng Jiao, Anhui Agricultural University, Hefei, China
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