ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Cell Biology
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1609413
This article is part of the Research TopicCell Development and Environmental Adaptation of Higher PlantsView all 4 articles
Early Protoplast Culture and Partial Regeneration in Cannabis sativa: Gene Expression Dynamics of Proliferation and Stress Response
Provisionally accepted
- Palacký University, Olomouc, Olomouc, Czechia
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Cannabis sativa L. is a plant of significant interest due to its high content of biologically active compounds, durable fibers, and bioeconomic potential. Despite recent progress in protoplast isolation, complete plant regeneration from cannabis protoplasts remains unachieved, highlighting gaps in protoplast-to-plant systems. This study reports the second successful establishment and partial regeneration of cannabis protoplast cultures, and investigates their molecular dynamics, marking a significant step forward. We demonstrated that the age of donor material is critical for the protoplast isolation, with the optimal source being 1-2-week-old leaves from in vitro-grown seedlings. Cultivation in a modified medium developed for Arabidopsis thaliana supported initial cell divisions and microcallus formation. Transcriptomic analyses of cell proliferation and stress response markers indicate that the cultured protoplasts were viable, re-entered the cell cycle, and exhibited oxidative and abiotic stress resilience. These findings enhance our understanding of cannabis cell biology and lay the groundwork for a protoplast-based regeneration system, paving the way for advanced applications in biotechnology.
Keywords: Protoplast isolation, Cell Proliferation, Gene Expression, stress response, Microcallus formation, Cannabis culture, Oxidative Stress, in vitro
Received: 10 Apr 2025; Accepted: 19 May 2025.
Copyright: © 2025 Král, Šenkyrík and Ondřej. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Daniel Král, Palacký University, Olomouc, Olomouc, Czechia
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