ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Genetics, Epigenetics and Chromosome Biology
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1610059
Comprehensive Genome-Wide Identification and Functional Analysis of the GT8 Gene Family in Eucalyptus Grandis
Provisionally accepted
- South China Agricultural University, Guangzhou, China
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The Glycosyltransferase 8 (GT8) family plays a crucial role in plant cell wall synthesis; however, there is significant functional differentiation among its members.Therefore, analyzing the members of the GT8 in woody plants is of great importance.In this study, various bioinformatics tools were employed to extract data from the whole genome database of Eucalyptus grandis. A systematic analysis was conducted on the classification of GT8 family members, physicochemical properties, subcellular localization, gene structure, chromosome location, and promoter information, with the aim of elucidating the function of the GT8 genes in E. grandis. We identified 52 GT8 family members in the E. grandis genome. Phylogenetic analysis classified these genes into four subfamilies: GAUT, GATL, GolS and PGSIP. The proteins' molecular weights varied from 15.75 to 185.00 kD, with an average molecular weight of 49.08 kD. These members were dispersed across chromosomes, with no distribution on chromosomes 3 and 7. Promoter analysis revealed that the majority of the GT8 family genes contained light-responsive elements, and all members included hormone-response cis-acting elements. Phylogenetic analysis suggests that EgGUX02 and EgGUX04 may be involved in the synthesis of xylan side chains by incorporating glucuronic acid (GlcA) into these structures. Likewise, it is probable that EgGAUT1 and EgGAUT12 directly contribute to the production of xylan and pectin. This study provides important insights into the molecular mechanisms underlying the synthesis of cell wall components, such as xylan and pectin, in E. grandis, contributing to a better understanding of the GT8 gene family.
Keywords: Glycosyltransferase1, GT82, Gene family3, Eucalyptus grandis4, 2 Functional analysis5
Received: 11 Apr 2025; Accepted: 31 May 2025.
Copyright: © 2025 Jie, Liu, Li, Wu, Fan and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Siwen Liu, South China Agricultural University, Guangzhou, China
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