ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Functional and Applied Plant Genomics
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1610315
This article is part of the Research TopicMolecular Selection of Promising Resistant Genotypes to Disease in Cereal CropsView all 4 articles
Transcriptome analysis of rice resistant and susceptible near-isogenic lines in response to infection by Xanthomonas oryzae pv. oryzicola
Provisionally accepted- State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, Guangxi University, Nanning, China
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Rice bacterial leaf streak (BLS), caused by gram-negative bacterium Xanthomonas oryzae pv. oryzicola (Xoc), is one of the most destructive quarantine diseases internationally. Effectively utilizing BLS resistance genes from wild rice to breed resistant varieties can solve the problem of controlling BLS at its source. In this study, resistant near-isogenic line NIL-bls2 (abbreviated as R) and susceptible near-isogenic line NIL-BLS2 (abbreviated as S) in BC4F3 were constructed by using Guangxi common wild rice material DY19, which carries the BLS resistance gene bls2 and susceptible indica rice variety 9311. Transcriptome sequencing was used to analyze the molecular interaction mechanism of R and S in response to infection by a highly pathogenic Xoc strain gx01. The results showed that between R and S, there were 218 differentially expressed genes (DEGs) at 12 hours post inoculation (hpi), 170 DEGs at 24 hpi, and 329 DEGs at 48 hpi after inoculation. GO and KEGG enrichment analysis revealed that the following changes occurred in R compared to S after Xoc infection: At 12 hpi, R enhanced cell wall toughness by synthesizing lignin; increased the ability to recognize and bind bacterial flagellin flg22, activating multiple immune responses of downstream signal transmission; and promoted wound healing by enhancing the synthesis of traumatic acid. At 24 hpi, R synthesized a large number of diterpenoid phytoalexins, up-regulated genes related to disease resistance protein PR1 and heat shock protein HSP90B, and activated jasmonic acid and salicylic acid-dependent signal transduction pathways. At 48 hpi, R carried out a defense reaction by strengthening the cell wall, enhancing jasmonic acid synthesis, synthesizing monoterpenes and isoquinoline alkaloids, etc. Taken together, bls2 was proposed to regulate both PTI-and ETI-related genes through multi-level defense system, including plant hormone-mediated regulation, antimicrobial phytoalexin biosynthesis, and structural barrier reinforcement,to against Xoc infection. It laid a theoretical foundation for further in-depth research into the molecular mechanism of the BLS resistance gene bls2.
Keywords: rice, Bacterial leaf streak (BLS), Transcriptome sequencing, Differentially Expressed Genes (DEGs), Near-isogenic line
Received: 11 Apr 2025; Accepted: 01 Sep 2025.
Copyright: © 2025 Zhang, Tang, Wan, Deng, Qin, Huang, Wei, Li and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Fang Liu, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, Guangxi University, Nanning, China
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