BRIEF RESEARCH REPORT article
Front. Plant Sci.
Sec. Plant Pathogen Interactions
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1616881
This article is part of the Research TopicInterplay of Plant Volatiles in Enhancing Immunity and Sustainable Pest ManagementView all 11 articles
Temporal Sampling of Root Exudates Using Coated Blade-SPME for Decoding Plant– Pathogen Interactions
Provisionally accepted
- 1London Research and Development Centre, Agriculture and Agri-Food Canada (AAFC), London, Canada
- 2Department of Biology, Western University, London, Ontario, Canada
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Root exudates shape the rhizosphere and mediate plant–microbe interactions, yet traditional sampling techniques often disturb the natural environment. Here, we present the use of coated blade-solid-phase microextraction (CB-SPME) method for passive, non-invasive in situ temporal metabolomic profiling of pea (Pisum sativum) root exudates during infection by the soil-borne pathogen Aphanomyces euteiches. In comparison to previously established extraction techniques, CB-SPME delivered lower absolute recovery but superior reproducibility while maintaining sensitivity. This non-destructive approach preserves rhizosphere integrity, enabling continuous monitoring of dynamic metabolite fluctuations and offers new insights into how root exudate influences plant–microbiome interactions.
Keywords: root exudate, Metabolomics, passive sampling, coated blade-solid phase microextraction, Solid Phase Microextraction, Pisum sativum, Aphanomyces root rot, Aphanomcyes euteiches
Received: 23 Apr 2025; Accepted: 28 Aug 2025.
Copyright: © 2025 Halane, McDowell and Dhaubhadel. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Sangeeta Dhaubhadel, London Research and Development Centre, Agriculture and Agri-Food Canada (AAFC), London, Canada
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