ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Metabolism and Chemodiversity
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1618168
Analysis of the main components of LSPN based on broadly targeted metabolomics technology
Provisionally accepted
- 1Provincial Key Laboratory of Veterinary Bio-pharmaceutical High tech Research, Jiangsu Agri-animal Husbandry Vocational College, Taizhou, China
- 2Yangzhou University, Yangzhou, Jiangsu Province, China
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Abstract: This study aimed to (1) establish a broadly targeted metabolomics workflow to evaluate batch-to-batch variability in Panax notoginseng-derived Panax 14 notoginseng stem/leaf-derived oral liquid (LSPN) preparations, (2) identify critical process-induced metabolite differences (e.g., saponin degradation products), and (3) 15 correlate these differences with pharmacopoeial quality markers (e.g., Rg1/Rb1 ratios). Our hypothesis was that thermal extraction parameters would significantly alter 16 saponin profiles, detectable via orthogonal metabolomic approaches (HPLC-MS/MS, PCA).The results showed a strong positive correlation (pearson correlation 17 coefficient >0.92) between triterpenoid saponins in LSPN-S and LSPN-C. Thirty-eight triterpenoid saponins were detected, of which 11 were up-regulated, 5 were 18 down-regulated, and the other 22 had less than a two-fold difference in content. Ginsenoside Rb3 showed a modest but significant downregulation in LSPN-S (fold change = 19 0.45, VIP = 1.11) compared with LSPN-C. Metabolomic analysis confirmed the suitability of the selected Panax notoginseng stems/leaves as raw materials, with 20 comprehensive characterization of LSPN's primary components.
Keywords: Targeted metabolome, Panax notoginseng stems and leaves, Biological activity, Main components, Metabolomics technology
Received: 02 May 2025; Accepted: 30 Sep 2025.
Copyright: © 2025 Qin, Chen, Wang, Li and Zhu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Feng Qin, qinancy922@163.com
Shanyuan Zhu, jstzmy922@163.com
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