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ORIGINAL RESEARCH article

Front. Plant Sci.

Sec. Plant Physiology

Volume 16 - 2025 | doi: 10.3389/fpls.2025.1622785

Integration of transcriptome and phytohormone analyses reveals antagonistic interactions between jasmonate and auxin signaling pathways in rice (Oryza sativa L.)

Provisionally accepted
Xiaoying  WuXiaoying Wu1*Yuting  CaoYuting Cao1Yeye  ChenYeye Chen1Wei  XiongWei Xiong1Gaorong  CaoGaorong Cao1Yan  ZengYan Zeng1Liqin  ZhangLiqin Zhang1Rensen  ZengRensen Zeng2Dongmei  ChenDongmei Chen2*
  • 1Huzhou University, Huzhou, China
  • 2Fujian Agriculture and Forestry University, Fuzhou, China

The final, formatted version of the article will be published soon.

Interactions between different hormones plays a central role in plant trade-off between growth and defense. Auxin is a pivotal growth hormone and jasmonates (JA) are key hormones for plant responses to environmental stressors. In this study we examined the interactions between auxin and JA in rice (Oryza sativa L.) by integration of transcriptome and phytohormone analyses. Exogenous application of methyl jasmonate (MeJA) to rice leaves led to markedly increased accumulation of jasmonic acid (JA), MeJA, and dihydrojasmonic acid (DJA) in both leaves and roots, as well as N-jasmonic acid isoleucine (JA-Ile) in the leaves. Importantly, MeJA application significantly influenced auxin biosynthesis and orchestrated large-scale changes in transcriptional regulation of L-tryptophan (Trp) biosynthesis, as well as indole-3-acetic acid (IAA) biosynthesis, catabolism, signaling transduction, and transport in rice leaves and roots, leading to notably decreased IAA in the leaves and roots, but increased levels of indole-3-carboxylic acid (ICA) and indole-3-carboxaldehyde (ICAld) in the leaves. Our findings suggest that JA signaling suppresses auxin signaling by reducing Trp flux into the indole-3-pyruvic acid (IPA) pathway, while enhancing Trp flux into the tryptamine (TAM) pathway, thereby fine-tuning rice growth upon JA burst.

Keywords: Jasmonate signaling, auxin, indole-3-acetic acid, Transcriptome, Antagonistic interaction, hormone crosstalk

Received: 04 May 2025; Accepted: 03 Sep 2025.

Copyright: © 2025 Wu, Cao, Chen, Xiong, Cao, Zeng, Zhang, Zeng and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Xiaoying Wu, Huzhou University, Huzhou, China
Dongmei Chen, Fujian Agriculture and Forestry University, Fuzhou, China

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