ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Metabolism and Chemodiversity
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1627126
This article is part of the Research TopicMolecular Architects of the Green World: Genetic, Epigenetic, and Transcriptional Regulation of Plant Metabolism and Chemo-diversityView all 5 articles
Comprehensive analysis of alternative splicing in Rosa roxburghii Tratt revealed its role in flavonoid synthesis
Provisionally accepted
- Moutai Institute, Zunyi, China
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Alternative splicing (AS) plays an important role in the synthesis of plant metabolites.Chestnut rose is a fruit with rich flavonoids and health benefits. However, the role of AS in its flavonoids synthesis is rarely reported. In this study, the AS landscape was characterized in different tissues of chestnut rose. The data showed that 8,586 genes could undergo AS and a total of 49,523 AS events were generated. Among them, tissuespecific AS genes were found in leaves, flowers and fruits. The content of flavonoids in the samples was detected and WGCNA analysis was performed with the AS genes.Five key modules of AS genes related to flavonoid synthesis were identified, and 4CL, ANR, DFR, MYB and other AS genes were validated by PCR and sequencing. In addition, qRT-PCR analysis revealed that the expression level of bHLH transcription factor AS transcript was higher than its full-length transcript, and it was highly expressed in FR1 and significantly correlated with flavonoids. Our research identified AS events in different tissues of chestnut rose and revealed their important functions in flavonoid synthesis. This study provides the basis for the molecular mechanism of flavonoids in chestnut rose.
Keywords: Alternative Splicing, Flavonoid synthesis, Rosa roxburghii tratt, molecular mechanism, WGCNA
Received: 15 May 2025; Accepted: 17 Jun 2025.
Copyright: © 2025 An, Wu, Chen and Shize. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Li Shize, Moutai Institute, Zunyi, China
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