ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Pathogen Interactions
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1629283
OsLB2.2 negatively regulates rice disease resistance at seedling stage in rice
Provisionally accepted
Tianze Ma1
Mengge Wang1Shupeng Xie1Chengxin Li2
Jingguo Wang1Hualong Liu1
Luomiao Yang1Longnan Men3Zhonghua Sun3Tianpeng Zong3
Detang Zou1
Hongliang Zheng1*Wei Xin1*- 1Northeast Agricultural University, Harbin, China
- 2Harbin Academy of Agricultural Sciences, Harbin, China
- 3Suihua Branch of Heilongjiang Academy of Agricultural Sciences, Suihua, China
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Background: Rice blast disease, caused by the fungal pathogen Magnaporthe oryzae, stands as the most destructive diseaset of rice, negatively impacting yield and quality. Identification of blast resistance genes is crucial for breeding disease resistant varieties. In this study, we conducted a genome-wide association analysis on rice blast with 295 Japonica rice varieties and the rice blast dominant physiological race ZD5 from Heilongjiang.Results: A total of 11 Quantitative Trait Loci (QTLs) encompassing 233 genes were mapped.Notably, 40 genes showed significant phenotypic variations among different haplotypes. By combining GWAS and RNA sequencing analysis, we identified five candidate genes related to rice seedling resistance to rice blast. Through the assessment of relative gene expression levels in 10 susceptible and 10 resistant varieties, OsLB2.2 (LOC_Os02g57470) emerged as a key gene displaying significant expression differences in resistant varieties under uninoculated and inoculated conditions. To elucidate gene functionality, we generated oslb2.2 knockout mutants.The results showed that oslb2.2 knockout mutants were significantly enhanced rice blast resistance compared to the wild type. Furthermore, we identified the dominant haplotype of 2 OsLB2.2 and developed Kompetitive Allele -Specific PCR (KASP) molecular markers for molecular improvement of rice blast resistance.The phenotypic validation indicated that OsLB2.2 negatively regulated the rice blast resistance at the seedling stage. Moreover, KASP molecular markers were developed, providing a theoretical basis for the potential application of OsLB2.2 in molecular breeding strategies.
Keywords: rice, blast, Genome-Wide Association Study, RNA-Seq, resistance gene
Received: 15 May 2025; Accepted: 16 Jul 2025.
Copyright: © 2025 Ma, Wang, Xie, Li, Wang, Liu, Yang, Men, Sun, Zong, Zou, Zheng and Xin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Hongliang Zheng, Northeast Agricultural University, Harbin, China
Wei Xin, Northeast Agricultural University, Harbin, China
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