METHODS article
Front. Plant Sci.
Sec. Technical Advances in Plant Science
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1630162
This article is part of the Research TopicPlant Phenotyping for AgricultureView all articles
Drone methods and educational resources for plant science and agriculture
Provisionally accepted- University of California, Davis, Davis, United States
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Technological advances have made drones (UAVs) increasingly important tools for the collection of trait data in plant science. Many costs for the analysis of plant populations have dropped precipitously in recent decades, particularly for genetic sequencing. Similarly, hardware advances have made it increasingly simple and practical to capture drone imagery of plant populations. However, converting this imagery into high-precision and high-throughput tabular data has become a major bottleneck in plant science. Here, we describe high-throughput phenotyping methods for the analysis of numerous plant traits based on imagery from diverse sensor types. Methods can be flexibly combined to extract data related to canopy temperature, area, height, volume, vegetation indices, and summary statistics derived from complex segmentations and classifications. We then describe educational and training resources for these methods, including a web page (PlantScienceDroneMethods.github.io) and an educational YouTube channel (https://www.youtube.com/@travisparkerplantscience) with step-by-step protocols, example data, and example scripts for the whole drone data processing pipeline. These resources facilitate the extraction of high-throughput and high-precision phenomic data, removing barriers to the phenomic analysis of large plant populations.
Keywords: UAV, UAS, QGIS, multispectral, thermal, RGB, high-throughput phenotyping, Phenomics
Received: 17 May 2025; Accepted: 16 Jul 2025.
Copyright: © 2025 Parker, Celebioglu, Watson and Gepts. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Travis Parker, University of California, Davis, Davis, United States
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