ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Abiotic Stress
This article is part of the Research TopicEnhancing Crop Resilience to Salt StressView all 12 articles
iTRAQ-Based Proteomic Profiling of Salt-Tolerant and Salt-Sensitive Potato (Solanum tuberosum) Cultivars under Salinity Stress
Provisionally accepted
- 1Fujian Agriculture and Forestry University, Fuzhou, China
- 2Xiamen University, Xiamen, China
- 3Qinghai University, Xining, China
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Soil salinity causes yield loss at harvest and quality deterioration during storage for cultivated potatoes (Solanum tuberosum). In this study, Based on previous studies on germplasm screening and physiological characteristics of salt-tolerant potato, as well as transcriptome analysis of potato root response to salt stress, two contrasting potato cultivars (the salt-tolerant potato cultivar M5008 and the salt-sensitive potato cultivarD516) were selected and exposed to the salt condition. Then, the protein expression profiles of these two cultivars were analyzed using an iTRAQ (isobaric tags for relative and absolute quantification)-based quantitative proteomic approach. Results showed that significant differences of 511 and 456 proteins in total were identified in the roots of D516 and M5008 cultivars, respectively. These differentially accumulated proteins (DAPs) were associated with redox homeostasis, sugar signaling and metabolism, osmotic metabolism, and phytohormone signaling pathways. Additionally, glucose metabolic (cluster 1), translational initiation (cluster 2), small molecule biosynthetic (cluster 3), ubiquitin-dependent protein catabolic (cluster 4), intracellular transport (cluster 5), and phosphatidylcholine metabolic (cluster 6) were the six largest functional clusters identified using protein-protein interaction network analysis. Among these DAPs, the expression proteins of G6PD1, P5CSA, PP2A2, TPS1, GAPCP1, and HEXO1 were associated with salt stress and verified at the transcriptional level using quantitative real-time PCR. Overall, the resulting data provide insight into the salt tolerance mechanisms of cultivars-specific-induced protein differences in potato cultivars.
Keywords: Solanum tuberosum, Soil salinity, iTRAQ-based quantitative proteomics, Differentially expressed proteins, protein-protein interaction
Received: 22 May 2025; Accepted: 05 Nov 2025.
Copyright: © 2025 Zhang, Wang, Zhao, Chen, Huang, Na and Guo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Tiancang Na, natiancang@163.com
Yuchun Guo, ycguo168@fafu.edu.cn
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