ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Pathogen Interactions
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1636064
The Application of Bacillus amyloliquefaciens and Arbuscular Mycorrhizal Fungi Displays Curative Effects on Citrus Huanglongbing
Provisionally accepted
- South China Agricultural University, Guangzhou, China
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Huanglongbing (HLB) is a severe citrus disease that results in significant yield loss, caused by the phloem-dwelling pathogen Candidatus Liberibacter asiaticus (CLas). Currently, antibiotics including penicillin, chloromycetin, and streptomycin are extensively utilized for the control of HLB in citrus production. However, various issues have emerged following the application of antibiotics, including the development of resistant microorganisms in soil and the accumulation of antibiotic residues in fruits. Consequently, there is an urgent need for alternative environmentally friendly methods for HLB control. In this study, the curative effects of Bacillus amyloliquefaciens strain HN11 and Arbuscular Mycorrhizal Fungi (AMF) on HLB were investigated. The HN11 strain and AMF demonstrated stable colonization within the interior of citrus roots. Moreover, HN11+AMF inoculation promoted the growth of HLB-infected citrus trees, accompanied by the decrease in CLas titers, reduction in H2O2 content, callus deposition, starch content, and ion leakage, as well as improvements in antioxidant enzyme activity indicators. The curative effects of HN11+AMF inoculation on HLB were also confirmed through field efficacy experiments. Moreover, HN11+AMF inoculation was identified to modify the composition and functions of root-soil bacterial and fungal microbial communities. These findings indicate that the use of HN11+AMF inoculation may serve as a promising control strategy for HLB management.
Keywords: Citrus HuangLongBing, Bacillus amyloliquefaciens, arbuscular mycorrhizal fungi, Double inoculation, plant-pathogen interactions
Received: 27 May 2025; Accepted: 11 Aug 2025.
Copyright: © 2025 Li, Lin, Liu, Zeng and Xu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Hanhong Xu, South China Agricultural University, Guangzhou, China
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