ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Functional and Applied Plant Genomics
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1637143
This article is part of the Research TopicGenomic Pathways to Plant Health: Exploring Microbial Symbiosis and BiocontrolView all articles
Metagenomic analysis of the effects of plant-and yeast-based formulations on the grapevine leaf microbiome of cv. 'Touriga Franca'
Provisionally accepted
Eliana Monteiro1,2
Paula Baptista3
Sofia Silva3
Márcia Carvalho1,2,4Radek Bragança5
Isabel Cortez1,2,6
Berta Gonçalves1,2,7
Isaura Castro1,2,4*- 1Centre for the Research and Technology of Agro-Environmental and Biological Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
- 2Institute for Innovation, Capacity Building and Sustainability of Agri-food Production, University of Trás-os-Montes e Alto Douro, Vila Real, Portugal
- 3Centro de Investigacao de Montanha, Bragança, Portugal
- 4Department of Genetics and Biotechnology, University of Trás-os-Montes e Alto Douro, Vila Real, Portugal
- 5Bangor University BioComposites Centre, Bangor, United Kingdom
- 6Department of Agronomy, University of Trás-os-Montes e Alto Douro, Vila Real, Portugal
- 7Department of Biology and Environment, University of Trás-os-Montes e Alto Douro, Vila Real, Portugal
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Grapevine leaf microbiome plays a crucial role in plant health and disease resilience. This study evaluated the impact of alternative plant-and yeast-based formulations compared to conventional grapevine diseases chemical treatments on the diversity and composition of leaves leaf fungal and bacterial communities over two consecutive years. High-throughput sequencing revealed that bacterial communities were more sensitive to treatments and variability by year variation compared to fungal communities, which remained stable. Conventional treatment (CT) showed the highest influence on fungal and bacterial composition, reducing the diversity of both. Some important fungal (Aureobasidium and Sporobolomyces) and bacterial genera (Pseudomonas and Sphingomonas) genera associated with the promotion of plant growth, health, and biocontrol were detected. These findings reinforce the potential of new treatments (nettle extract, Japanese knotweed extract and yeast-based formulation -T66) with a putative fungicide effects and positive effects on beneficial microbial species. These treatments can to modulate the leaf microbiome, particularly bacterial communities, without disrupting the natural fungal balance, thus., These highlighting its their relevance in thefor development developing of sustainable viticultural practices that aimaimed to at improve improving plant protection.
Keywords: Leaves microbiome, Microbial community diversity, metabarcoding, Plant Extracts, Vitis vinifera L., yeast-based formulations
Received: 28 May 2025; Accepted: 28 Jul 2025.
Copyright: © 2025 Monteiro, Baptista, Silva, Carvalho, Bragança, Cortez, Gonçalves and Castro. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Isaura Castro, Centre for the Research and Technology of Agro-Environmental and Biological Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
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