BRIEF RESEARCH REPORT article
Front. Plant Sci.
Sec. Aquatic Photosynthetic Organisms
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1640158
Systems metabolic engineering of Escherichia coli for the bioproduction of biliverdin and phycoerythrobilin
Provisionally accepted
- 1Institute of Oceanology, Chinese Academy of Sciences (CAS), Qingdao, China
- 2Yantai University, Yantai, China
- 3Ocean University of China, Qingdao, China
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Phycobiliprotein is an important co-pigment in photosynthesis, which is composed of the covalent combination of apoprotein and phycobilin. Biliverdin IXα and phycoerythrobilin are both important substances in the phycobiliprotein biosynthesis pathway. As an economic red seaweed, Neoporphyra haitanensis has a high content of phycoerythrin. Therefore, in this study, we explored new enzyme resources for the heterologous biosynthesis of biliverdin and phycoerythrobilin. Specifically, we identified and isolated the genes encoding NhHO1, NhPebA and NhPebB from N. haitanensis, which are integral components of its phycoerythrin biosynthetic pathway.Additionally, ApHO1 from Arthrospira platensis and PebS from Prochlorococcus phage P-SSM2 were selected for comparative analysis. The results showed that genes from N. haitanensis did not encode active enzymes, which might be ascribed to the absence of crucial motifs (motif 5 and motif 10). However, the transformation of ApHO1 and PebS into Escherichia coli could lead to the synthesis of biliverdin and phycoerythrobilin. This is the first report of sequence analysis and enzyme activity verification of phycoerythrin synthesis genes from N. haitanensis, providing a foundation for future explorations into its potential genetic resources. The successful production of biliverdin and phycoerythrobilin lay a foundation for the environmentally friendly preparation of phycobiliprotein.
Keywords: Biliverdin, Phycoerythrobilin, heterologous expression, biosynthesis, Neoporphyra haitanensis
Received: 03 Jun 2025; Accepted: 02 Jul 2025.
Copyright: © 2025 Li, Lu, Zang, Duan and Shao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Delin Duan, Institute of Oceanology, Chinese Academy of Sciences (CAS), Qingdao, China
Zhanru Shao, Institute of Oceanology, Chinese Academy of Sciences (CAS), Qingdao, China
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