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ORIGINAL RESEARCH article

Front. Plant Sci.

Sec. Plant Metabolism and Chemodiversity

Volume 16 - 2025 | doi: 10.3389/fpls.2025.1640426

Development of plant extracts as substrates for untargeted transporter substrate identification in Xenopus oocytes

Provisionally accepted
Christos TheodorouChristos Theodorou1Victor de-Prado-ParralejoVictor de-Prado-Parralejo1Deyang XuDeyang Xu1Yoshimasa TodorokiYoshimasa Todoroki2Louise SvenningsenLouise Svenningsen1Tetsuya MoriTetsuya Mori3Christoph CrocollChristoph Crocoll1Masami Yokota HiraiMasami Yokota Hirai3Hiroshi TsugawaHiroshi Tsugawa2,3,4Hussam Hassan Nour-EldinHussam Hassan Nour-Eldin1Barbara Ann HalkierBarbara Ann Halkier1*
  • 1DynaMo Center, Department of Plant and Environmental Sciences, Frederiksberg, Denmark
  • 2Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Japan
  • 3RIKEN Center for Sustainable Resource Science, Yokohama, Japan
  • 4RIKEN Center for Integrative Medical Sciences, Yokohama, Japan

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• Development of a two-phase liquid-liquid extraction method to generate metabolically diverse Arabidopsis thaliana seedling extracts. • Optimized extracts for high-throughput functional screening of plant transporters using Xenopus laevis oocytes. • Identified a protocol that minimizes oocyte membrane permeation while maximizing metabolite coverage and repeatability • Combined extracts from biotic (flagellin 22, chitin) and abiotic (phosphorus, nitrogen starvation) stress treatments to expand metabolite diversity. • Identified more than 200 primary and secondary metabolites based on a combinatorial technique

Keywords: non-targeted metabolomics, Mass Spectrometry, transportomics, Oocytes, transporters, Plant Extracts

Received: 03 Jun 2025; Accepted: 07 Aug 2025.

Copyright: © 2025 Theodorou, de-Prado-Parralejo, Xu, Todoroki, Svenningsen, Mori, Crocoll, Hirai, Tsugawa, Nour-Eldin and Halkier. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Barbara Ann Halkier, DynaMo Center, Department of Plant and Environmental Sciences, Frederiksberg, Denmark

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Supplementary Material