ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Breeding
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1641401
Glutathione S-transferase CrGST24 in the differentiation of adventitious buds from Camellia reticulata callus
Provisionally accepted
- Southwest Forestry University, Kunming, China
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Camellia reticulata holds cultural and horticultural significance in traditional Chinese gardens, as a regional endemic species in Yunnan Province. However, during the in vitro regeneration process of C. reticulata, there is often a phenomenon of low efficiency of adventitious bud differentiation or no adventitious bud differentiation at all. In previous study, we observed significant morphological differences between the callus tissues of C. reticulata 'Zipao' and wild species. The callus of 'Zipao' was white and loosely textured, the wild species was green and hard. To investigate the differences between these two types of callus, we conducted transcriptome analysis and identified a differentially expressed gene GST24, which is closely related to the synthesis of glutathione (GSH). Heterologous transformation of CrGST24 into tobacco (Nicotiana tabacum) was conducted. The CrGST24 gene promoted the synthesis of endogenous auxin and cytokinin in transgenic tobacco by regulating the expression of transcription factors related to auxin and cytokinin. Exogenous IBA, 6-BA, and red-blue light treatment increased the levels of auxins and cytokinins in C. reticulata callus, thereby promoting adventitious bud differentiation. Additionally, CrGST24 interacted with CrGSHB and CrDHAR2 genes, facilitating GSH and AsA synthesis and clearing ROS.
Keywords: Camellia reticulata, CrGST24, auxin, Cytokinin, GSH, ASA, ROS
Received: 05 Jun 2025; Accepted: 24 Jul 2025.
Copyright: © 2025 Hang, Zhao, Kai, Xian, Cheng, Ting and Tian. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Wu Tian, Southwest Forestry University, Kunming, China
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