ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Functional and Applied Plant Genomics
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1641897
Identification and Expression Analysis of the BnE2F/DP Gene Family in Brassica napus
Provisionally accepted
- 1Leshan Normal University, Leshan, China
- 2Changzhi Vocational and Technical College, Changzhi, China
- 3Southwest University, Chongqing, China
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Identify the members of the E2F/DP (Eukaryotic Transcription Factor 2/Dimerization Partner) gene family in Brassica napus and elucidate the gene functions and tissue expression patterns of the BnE2F/DP gene family. Utilize bioinformatics approaches to identify the BnE2F/DP gene family in Brassica napus. Construct a phylogenetic tree for evolutionary relationship analysis, and analyze the chromosomal distribution, gene structure composition, types of cis-acting elements, as well as intragenomic and interspecific collinearity. Integrate transcriptome data with real-time quantitative PCR (RT-qPCR) technology to explore the expression patterns of BnE2F/DP gene family members across various tissues. Total 29 BnE2F/DP genes were identified in Brassica napus and classified into 8 sub-families. These genes were unevenly distributed across 15 chromosomes, with enrichment on chromosomes 3 and 13. Intragenomic collinearity analysis detected 35 pairs of collinear BnE2F/DP gene pairs. Interspecific analysis revealed that there were 9 pairs of orthologous gene pairs between Brassica napus and Arabidopsis thaliana. Cis-acting element analysis showed that members of the BnE2F/DP family harbored four types of cis-acting elements, which might be involved in multiple regulatory processes, including hormonal regulation, abiotic stress responses, light reactions, and growth and development. Transcriptome and quantitative PCR data analyses indicate that members of the E2FC subfamily are likely to actively regulate seed and embryo development and positively respond to various abiotic stresses.. The conserved motifs and gene structures within each BnE2F/DP subfamily are largely consistent, which indicates that BnE2F/DP plays a crucial role in the growth and development of rapeseed seeds and embryos, as well as in the response to various abiotic stresses.
Keywords: Brassica napus, E2F/DP, gene family, expression analysis, bioinformatics
Received: 06 Jun 2025; Accepted: 30 Jul 2025.
Copyright: © 2025 Fu, Min and Lijuan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Chun Fu, Leshan Normal University, Leshan, China
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