ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Pathogen Interactions
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1652068
Proteomic Analysis of Rice Mutant pir1 Reveals Molecular Mechanisms Triggering PCD and Conferring High Resistance to Bacterial Blight
Provisionally accepted
- 1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products and Institute of Agro-product Safety and Nutrition; Zhejiang Academy of Agricultural Sciences, Hangzhou, China
- 2Shenyang Agricultural University, Shenyang, China
- 3Zhuji Agricultural Technology Extension Center, Zhuji, China
- 4Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, China
- 5Zhejiang Academy of Agricultural Sciences, Hangzhou, China
- 6Taizhou Academy of Agricultural Sciences, Taizhou, China
- 7Wuwangnong Seed shareholding Co., Ltd., Hangzhou, China
- 8National Key Laboratory for Development and Utilization of Forest Food Resources, Hangzhou, China
- 9Qujiang District Agricultural Technology Extension Center, Quzhou, China
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Most rice mutants exhibit some level of resistance to bacterial blight. This study demonstrates that the rice lesion mimic mutant (LMM) pir1 possesses enhanced resistance to bacterial leaf blight and triggers the upregulation of multiple pathogenesis-related (PR) proteins. Concurrently, photosynthetic parameter measurements revealed a significant impairment in the photosynthetic electron transport chain and photosynthetic capacity in pir1. Assessments of various stress factors and electron microscopy observations indicated that accumulated reactive oxygen species (ROS) caused severe damage to plant organelles. Utilizing proteomic approaches, we analyzed differentially expressed proteins (DEPs) between pir1 and its wild-type counterpart. Two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) combined with mass spectrometry (MS) analysis of different leaf positions from both materials identified a total of 321 DEPs, comprising 87 upregulated and 234 downregulated proteins. Bioinformatics analysis of these DEPs revealed their involvement in diverse biological processes, including photosynthesis, carbohydrate metabolism, defense responses, redox homeostasis, and energy metabolism. Analysis of the regulatory network suggests that the mutation pir1 participates in programmed cell death (PCD), thereby triggering disease resistance responses.
Keywords: Disease Resistance, Xoo, Proteomics, ROS, Photosynthetic pathways, Defense pathways
Received: 23 Jun 2025; Accepted: 05 Aug 2025.
Copyright: © 2025 Chen, Zhou, Liang, Zhou, Xie, Hou, Zheng and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Bingsong Zheng, National Key Laboratory for Development and Utilization of Forest Food Resources, Hangzhou, China
Jianzhong Li, Qujiang District Agricultural Technology Extension Center, Quzhou, China
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