ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Functional and Applied Plant Genomics
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1654390
This article is part of the Research TopicAnalysis of Genetic Basis of Crop Stress Resistance and Variety ImprovementView all 7 articles
Genome-Wide Identification and Expression Analysis of CCoAOMT Genes in Capsicum annuum L. Under Drought Stress
Provisionally accepted
- 1Chili Pepper Research Institute of Guizhou, Guiyang, China
- 2Guizhou Key Laboratory of Molecular Breeding for Characteristic Horticultural Crops, Guiyang, China
- 3Guizhou Mountain Agricultural Machinery Research Institute, Guiyang, China
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Caffeoyl-CoA O-methyltransferase (CCoAOMT) is a key enzyme in lignin biosynthesis. Based on the Zunla 1 genome, 11 CaCCoAOMT genes (CaCCoAOMT1-CaCCoAOMT11) were identified and analyzed for their physicochemical properties, phylogeny, gene structure, and expression patterns. qRT-PCR validated their drought-responsive expression, and subcellular localization of CCoAOMT1 and CCoAOMT2 was examined in tobacco leaves. The genes are unevenly distributed across chromosomes 1, 2, 4, and 8, with CaCCoAOMT10 and CaCCoAOMT11 unanchored. The proteins range from 143 to 380 amino acids and have 2-10 exons. Phylogenetic analysis grouped them into clades II, III, V, and VII. Conserved motif analysis revealed 10 motifs, with motifs 1 and 2 present in all genes. Promoter analysis indicated responsiveness to light, hormones, and drought stress. All genes except CaCCoAOMT6 showed tissue-and developmental stage-specific expression. Under drought stress, six genes were significantly downregulated and two were significantly upregulated in roots. CaCCoAOMT1 and CaCCoAOMT2 were localized in both the cytoplasm and nucleus. These findings provide insights into the functional roles and regulation of CaCCoAOMT genes in pepper under drought stress.
Keywords: Capsicum annuum, CCoAOMT gene family, Drought stress, Expression, whole-ge-nome identification
Received: 26 Jun 2025; Accepted: 29 Aug 2025.
Copyright: © 2025 Di, Lu, Bai, Yan, Lai, Zhang, He and He. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Wu Di, Chili Pepper Research Institute of Guizhou, Guiyang, China
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