ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Crop and Product Physiology
This article is part of the Research TopicAdvancements in Light Management Strategies for Crop ProductivityView all 9 articles
Light Spectrum Effects on Micropropagation and Gene Expression of Bucephalandra sp. in a Temporary Immersion System for Sustainable Production
Provisionally accepted
- 1Kasetsart University Department of Agronomy, Kamphaeng Saen, Thailand
- 2Burapha University Faculty of Agricultural Technology Sakaeo Campus, Sakaeo, Thailand
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Bucephalandra sp. 'Wavy Dark Green' is a commercially valuable ornamental aquatic plant with limited propagation potential due to low multiplication rates and acclimatization challenges. This study aimed to developed an integrated tissue culture protocol by optimizing disinfection procedures, basal media and plant growth regulator, temporary immersion system (TIS) conditions, light quality, and rooting and acclimatization methods. Surface sterilization using 0.1–0.2% HgCl₂ or a two-step 1.2% NaOCl protocol significantly improved explant survival and reduced contamination. MS medium supplemented with 5 mg/L benzylaminopurine (BA) produced the highest shoot proliferation on solid culture media. In TIS, six immersions per day for 2–3 minutes yielded optimal shoot multiplication and biomass. Blue:red (70:30) and pure blue light enhanced chlorophyll content, photosynthetic efficiency (QY), stomatal opening, and upregulated key light-responsive genes (psaA, psbB, petG, pskB, atpA, petA). Rooting was most effective in ¼ or full-strength MS medium with 0.3 mg/L naphthaleneacetic acid (NAA). Acclimatized plantlets grown in sand exhibited the highest survival and growth. This integrated protocol supports efficient micropropagation and ex vitro establishment of Bucephalandra sp. 'Wavy Dark Green', facilitating its conservation, ornamental use, and sustainable export, in line with SDG 12 (Responsible Consumption and Production).
Keywords: Aquatic plant, In vitro Propagation, Liquid medium, light regime, Photosynthesis efficiency, temporary immersion system, Bucephalandra, sustainable production
Received: 21 Jul 2025; Accepted: 19 Nov 2025.
Copyright: © 2025 Chanprame, Laksana, Sophiphun and Kaewhom. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Sontichai Chanprame, agrstc@ku.ac.th
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