ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Bioinformatics
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1661360
Expression of Recombinant Cap antigen of porcine circovirus type 2 in the chloroplast of Chlamydomonas reinhardtii
Provisionally accepted
- Zhejiang Sci-Tech University, Hangzhou, China
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The capsid (Cap) protein encoded by ORF2 gene of porcine circovirus type 2 is the major immunogen for development of vaccines and can effectively reduce the incidence of porcine circovirus-associated diseases. In order to explore an efficient expression mode of Cap protein, using Chlamydomonas reinhardtii chloroplast as the expression platform of Cap protein, the C. reinhardtii chloroplast expression vector pCR02 of the optimized ORF2 gene was constructed and transferred into C. reinhardtii chloroplast by biolistic bombardment method. After multiple rounds of resistance screening and culturing, PCR, RT-PCR, Western-blot and ELISA were used to detect the ORF2 gene and the expression of Cap protein in C. reinhardtii transformants. The results of the study showed that the ORF2 antigen gene had been correctly integrated into the specific site of the C. reinhardtii chloroplast genome and the Cap protein was effectively expressed. These results suggested that C. reinhardtii chloroplast is a potential platform for production of recombinant antigen proteins, and provided important support for exploring new production pathways of recombinant pharmaceutical protein.
Keywords: Porcine circovirus type 2, ORF2 antigen gene, capsid protein, Chlamydomonas reinhardtii, chloroplast transformation
Received: 07 Jul 2025; Accepted: 12 Sep 2025.
Copyright: © 2025 Song, Lin, Cheng, Li, Li and Yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Zongqi Yang, yangzongqi@zstu.edu.cn
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