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ORIGINAL RESEARCH article

Front. Plant Sci.

Sec. Functional and Applied Plant Genomics

Volume 16 - 2025 | doi: 10.3389/fpls.2025.1673408

Comprehensive Identification, Characterization, and Expression Analysis of m6A Methylation Transferase Gene Family in Maize (Zea mays L.)

Provisionally accepted
Chunlei  LiChunlei Li1Ming  HeMing He2Xinyi  LiuXinyi Liu1Qingxue  LvQingxue Lv2Xiaoming  YuXiaoming Yu1Xiaoxue  FanXiaoxue Fan2Xia  BaiXia Bai1Jie  CongJie Cong1*Chuanbo  SunChuanbo Sun2*
  • 1Jilin Agricultural Science and Technology University, Jilin, China
  • 2Jilin Academy of Agricultural Sciences Gongzhuling Branch, Gongzhuling, China

The final, formatted version of the article will be published soon.

N6-methyladenosine (m6A) RNA represents the most prevalent internal modification found in mRNA and plays a crucial role in stress response and developmental processes across various crop species. However, the understanding of m6A modification in monocot species remains limited. In this study, we conducted a comprehensive analysis of m6A methyltransferase genes "writers" in maize, focusing on gene localization, structural characteristics, conserved domains, phylogenetic relationships, promoter analysis, and expression profiles. Our analysis identified three m6A regulatory genes within the maize genome. Through the phylogenetic relationship analysis, we classified these genes into three distinct clusters alongside the model species Arabidopsis thaliana and Oryza sativa. Promoter analysis revealed that m6A pathway genes are mainly associated with hormone-responsive elements, environmental stress-related elements, and transcription factors. The conserved domain analysis indicated that all identified maize m6A proteins possess the MT-A70 domain. Furthermore, RNA-seq and RT-qPCR analysis demonstrated that the identified Zmm6A genes exhibit tissue-specific expression patterns, as well as differential expression in response to various abiotic stresses, suggesting a potential role for m6A modification in influencing reproductive development. Notably, the expression of ZmMTA-03 genes was significantly upregulated under cold stress conditions. This study provides valuable insights into the regulatory genes associated with m6A modification and their potential epigenetic regulatory mechanisms in maize.

Keywords: Maize, epitranscriptomic regulation, m6A modifier, evolution, expression profile, cis-acting elements

Received: 28 Jul 2025; Accepted: 06 Oct 2025.

Copyright: © 2025 Li, He, Liu, Lv, Yu, Fan, Bai, Cong and Sun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Jie Cong, congjie0703@outlook.com
Chuanbo Sun, jlsnkyyms@163.com

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