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ORIGINAL RESEARCH article

Front. Plant Sci.

Sec. Crop and Product Physiology

Volume 16 - 2025 | doi: 10.3389/fpls.2025.1681593

Grape berries biochemical shifts from vines under summer stress treated with kaolin and silicon mixtures

Provisionally accepted
  • 1University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
  • 2Universidade de Tras-os-Montes e Alto Douro Centro de Investigacao e de Tecnologias Agro-Ambientais e Biologicas, Vila Real, Portugal
  • 3Instituto Regional de Investigacion y Desarrollo Agroalimentario y Forestal de Castilla-La Mancha, Tomelloso, Spain
  • 4Universidade do Porto, Porto, Portugal
  • 5Universitat Jaume I, Castellón de la Plana, Spain

The final, formatted version of the article will be published soon.

Climate change is intensifying abiotic stress in viticulture, with higher temperatures, water deficits, and increased solar radiation directly affecting grape berry development, biochemical balance, and overall fruit quality. These challenges compromise the delicate balance of sugars, acids, phenolic compounds, and aromatic profiles that define the sensory attributes and typicity of the resulting wine. Innovative practices are therefore needed to sustain fruit quality and composition under increasingly variable and extreme climatic conditions. Silicon(Si) and kaolin(Kl) have already shown highly positive effects in mitigating the impacts of climate change on grapevines. Building on this, the aim of this study was to test their combined foliar application to simultaneously alleviate drought and heat stress and enhance berry quality. An integrated assessment was conducted over two growing seasons in a commercial vineyard (Vitis vinifera L. cv. Touriga Franca) located at Quinta de Ventozelo(Douro Demarcated Region, Portugal) to evaluate the effects of combined Kl(2%) and Si foliar sprays at different concentrations(2–8%). The study included analyses of fruit biochemical composition, must quality, cuticular wax profile, histological traits, carbon isotope discrimination (δ¹³C), hormonal balance, and yield parameters. Results showed that Si and Kl treatments modulated secondary metabolite accumulation (phenols, flavonoids, anthocyanins, and tannins) in a season-and stage-dependent manner, with significant increases under milder environmental conditions, particularly in seasons with lower heat and drought stress. This suggests that these products can act as elicitors or stress mitigators, depending on the environmental context. Treated vines maintained higher organic acid levels and lower probable alcohol content, indicating an improved sugar–acid balance. Moreover, the treatments influenced the cuticular wax composition, enhancing triterpenoid content and increasing cuticle thickness and epidermal cell size, which, together with enriched δ¹³C values, support improved water-use efficiency.The hormonal profiles confirmed the role of Si and Kl in fine-tuning stress and growth signals, contributing to better fruit robustness.These findings demonstrate that the combined foliar application of kaolin and silicon is a promising tool to protect grape berry quality by modulating biochemical composition, cuticular wax profile, histological traits, isotopic signature, and hormonal balance, helping maintain fruit integrity and quality, and compositional stability under the ongoing challenges of climate change.

Keywords: viticulture, Kaolin, Silicon, Abiotic stress mitigation, Secondarymetabolites, Grape berry quality, foliar application, Cuticular waxes

Received: 07 Aug 2025; Accepted: 02 Oct 2025.

Copyright: © 2025 Pereira, Baltazar, Branco, Marques Monteiro, Serrano, Morales, Cruz, Casal, Balfagón, Moura, Moutinho-Pereira and Dinis. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Sandra Pereira, sirp@utad.pt

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