ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Cell Biology
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1685260
This article is part of the Research TopicENPER2024 - Three Decades of Endomembrane ResearchView all articles
The fate and function of the Arabidopsis Class I formin AtFH1 during central vacuole biogenesis in the rhizodermis
Provisionally accepted
- Department of Experimental Plant Biology, Charles University, Prague, Czechia
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Introduction: During plant cell differentiation, major restructuring of endomembranes takes place, resulting in formation of the large central vacuole. We previously reported that the Arabidopsis Class I formin AtFH1 (At3g25500), a member of a plant-specific family of cytoskeletal organizers that are at the same time integral membrane proteins, transiently localizes to the tonoplast in the root transition zone around the time of central vacuole consolidation. Methods: We used in vivo fluorescent protein expression in combination with pharmacological treatments and quantitative image analysis to examine the behavior and role of AtFH1 during central vacuole formation. Results: The protein was found to enter vacuole lumen in the root elongation zone and some shoot tissues, suggesting that its brief residence at the tonoplast in the transition zone is also an intermediate of a process leading to its vacuolar degradation. However, impairment of AtFH1 function by mutations or by the formin inhibitor SMIFH2 resulted in altered vacuole organization, while only the loss of function mutations affected rapid, cytoplasmic streaming-related tonoplast motility. Discussion: These observations suggest that tonoplast-associated AtFH1 may act as an active cargo that affects tonoplast organization while on the way towards being degraded.
Keywords: At3g25500, Cytoplasmic Streaming, formin, fluorescent protein pH sensitivity, Protein degradation, Root tip, vacuole configuration, tonoplast
Received: 13 Aug 2025; Accepted: 29 Sep 2025.
Copyright: © 2025 Kocova, Zarsky and Cvrckova. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Fatima Cvrckova, fatima.cvrckova@natur.cuni.cz
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