ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Functional and Applied Plant Genomics
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1693512
This article is part of the Research TopicAnalysis of Genetic Basis of Crop Stress Resistance and Variety ImprovementView all 9 articles
Study on mechanism of Temperature-Modulated Polyphenolic Biosynthesis in Cigar Tobacco Leaves
Provisionally accepted
- 1Henan Agricultural University, Zhengzhou, China
- 2China Tobacco Sichuan Industrial Co Ltd, Chengdu, China
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Cigars as an economic crop, inappropriate growth temperature can detrimentally affect cigar quality, yet our knowledge about the response of cigar tobacco leaves (CTLs) to such stressors remains limited. This study revealed that both long-term mild low and high temperatures in CTLs triggered excessive production of reactive oxygen species (ROS) and disrupted antioxidant enzyme systems. Exposure to low temperatures resulted in increased accumulation of phenolic and flavonoids, while high temperatures were associated with either negative or negligible effects on these metabolites. Moreover, low temperatures significantly stimulated phenylpropanoid biosynthesis, in contrast to the negative effects observed at high temperatures. Correlation analysis indicated that NtMYB12-1, NtMYB12-2, and NtMYB12-3 likely acted as crucial regulators in the modulation of enzymes involved in phenylpropanoid biosynthesis under variable temperature conditions. Furthermore, weighted co-expression network analysis identified that NtCHS-6, Nt4CL-6, and NtPAL-1 worked as central hub genes in phenylpropanoid biosynthesis-related modules. This study provides a deeper understanding of the complex theoretical framework of phenylpropanoid biosynthesis in CTLs when exposed to fluctuating temperatures, offering valuable insights for the cultivation of high-quality cigar products .
Keywords: Cigar tobacco, Long-term, phenylpropanoid biosynthesis, RNA-Seq, Temperature stress, WGCNA
Received: 27 Aug 2025; Accepted: 09 Oct 2025.
Copyright: © 2025 Kang, Fu, Yan, Xiao, Zhao and Yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Mingqin Zhao, zhaomingqin@henau.edu.cn
Weili Yang, weiliyang2012@163.com
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