ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Abiotic Stress
This article is part of the Research TopicExploring Cold Tolerance and Stress in PlantsView all 3 articles
Compared Analysis of Physiology and Transcriptomics Reveals Superior Cold Tolerance in CV-1 Compared to K326
Provisionally accepted
- 1Guizhou Academy of Tobacco Sciences, Guiyang, China
- 2Central South University, Changsha, China
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Introduction: Low-temperature stress can cause damage to the growth and development of tobacco plants and the yield and quality of tobacco leaves. Methods: To elucidate the physiological and molecular mechanisms underlying the differing responses of different tobacco varieties to low-temperature stress at 6°C, transcriptomics analysis was employed to investigate the differences in physiological and gene regulatory networks between K326 and CV-1. Results: Morphological analysis revealed that CV-1 recovered more quickly to its pre-cold stress state than K326 during the later stages of low-temperature stress, demonstrating stronger cold tolerance. Physiological and biochemical analyses showed that compared to K326, CV-1 exhibited stronger antioxidant enzyme activities (superoxide dismutase, catalase, peroxidase) and lower membrane lipid peroxidation damage, as indicated by the decreased malondialdehyde content. Differential gene expression analysis indicated that the enhanced cold tolerance of CV-1 may be attributed to stronger phenylalanine synthesis capacity, NADH synthesis, and antioxidant enzyme activities. Weighted co-expression network analysis revealed that the enhanced cold tolerance of CV-1 may be attributed to its unique SUMOylation and phosphorylation regulatory pathways of proteins such as DeSI1L, EDS1L, and EXPA2L. Compared to K326 under low-temperature stress, CV-1 also exhibits stronger photosynthetic capacity, hydrogen peroxide transport capacity, and isoflavone synthesis capacity. Additionally, CV-1 shows higher expression of the SPFH protein superfamily and heat shock protein family. Discussion: This study revealed differences in gene regulatory networks between K326 and CV-1 in response to low-temperature stress and identified candidate genes associated with low-temperature stress, which can be utilized for genetic improvement of tobacco plants to enhance their cold tolerance.
Keywords: Physiology, Transcriptomics, Cold-treatment, Tobacco, Weighted gene co-, expression network analysis
Received: 13 Sep 2025; Accepted: 04 Nov 2025.
Copyright: © 2025 Yang, Wang, Luo, Yang, Zhu, Gan and Yu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Jianyu Zhu, zhujy@csu.edu.cn
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